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| Mapping technique for biodistribution of elements in a model organism, Caenorhabditis elegans, after exposure to copper nanoparticles with microbeam synchrotron radiation X-ray fluorescence | |
Gao YX(高愈希) ; Liu NQ(刘年庆); Chen CY(陈春英); Li YF(李玉锋); Zhang ZY(张智勇); Zhao YL(赵宇亮); Chai ZF(柴之芳); Gao, Y; Liu, NQ; Chen, CY; Luo, YF; Li, YF; Zhang, ZY; Zhao, YL; Zhao, BL; Iida, A; Chai, ZF
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| 2008 | |
| Source Publication | JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
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| Volume | 23Issue:8Pages:1121-1124 |
| Corresponding Author | [Gao, Yuxi ; Liu, Nianqing ; Chen, Chunying ; Li, Yufeng ; Zhang, Zhiyong ; Zhao, Yuliang ; Chai, Zhifang] Chinese Acad Sci, Bioenvironm Effects Nanomat & Nanosafety, Inst High Energy Phys, Beijing 100049, Peoples R China ; [Gao, Yuxi ; Liu, Nianqing ; Chen, Chunying ; Li, Yufeng ; Zhang, Zhiyong ; Zhao, Yuliang ; Chai, Zhifang] Chinese Acad Sci, Natl Ctr Nanosci & Technol China, Beijing 100049, Peoples R China ; [Luo, Yunfeng] Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China ; [Chen, Chunying] Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China ; [Iida, Atsuo] High Energy Accelerator Res Org, Photon Factory, Inst Mat Struct Sci, Tsukuba, Ibaraki 3050801, Japan |
| Subtype | Article |
| Abstract | To investigate the toxicological effects of nanomaterials, experimental studies on the absorption and accumulation in organisms are of broad interest. In the present study, Caenorhabditis elegans (C. elegans) was used as a "model'' organism to investigate the bioaccumulation and toxicological effects of engineered copper nanoparticles with a scanning technique of microbeam synchrotron radiation X-ray fluorescence (m-SRXRF). The adult hermaphrodite is anatomically simple with 959 somatic cells and 1 mm in length. The mapping results of the whole organism indicate that the exposure to copper nanoparticles can result in an obvious elevation of Cu and K levels, and a change of bio-distribution of Cu in nematodes. Accumulation of Cu occurs in the head and at a location 1/3 of the way up the body from the tail compared to the un- exposed control. In contrast, a higher amount of Cu was detected in other portion of worm body, especially in its excretory cells and intestine when exposed to Cu(2+). The results compared well with total Cu levels in nematodes, which were 4.10 +/- 0.54, 12.32 +/- 0.49 and 5.22 +/- 0.63 mu g g(-1) dry weight for the PBS, Cu(2+) and Cu nanoparticle groups, respectively, measured by ICP- MS. The nondestructive and multi- elemental m- SRXRF provides an important tool for mapping the elemental distribution in the whole body of a single tiny nematode at lower levels. |
| Subject Area | Chemistry; Spectroscopy |
| DOI | 10.1039/b802338g |
| URL | 查看原文 |
| Language | 英语 |
| WOS Research Area | Chemistry ; Spectroscopy |
| WOS Subject | Chemistry, Analytical ; Spectroscopy |
| WOS ID | WOS:000257982600012 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihep.ac.cn/handle/311005/239081 |
| Collection | 院士 加速器中心 多学科研究中心 |
| Affiliation | 中国科学院高能物理研究所 |
| First Author Affilication | Institute of High Energy |
| Recommended Citation GB/T 7714 | Gao YX,Liu NQ,Chen CY,et al. Mapping technique for biodistribution of elements in a model organism, Caenorhabditis elegans, after exposure to copper nanoparticles with microbeam synchrotron radiation X-ray fluorescence[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2008,23(8):1121-1124. |
| APA | 高愈希.,刘年庆.,陈春英.,李玉锋.,张智勇.,...&Chai, ZF.(2008).Mapping technique for biodistribution of elements in a model organism, Caenorhabditis elegans, after exposure to copper nanoparticles with microbeam synchrotron radiation X-ray fluorescence.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,23(8),1121-1124. |
| MLA | 高愈希,et al."Mapping technique for biodistribution of elements in a model organism, Caenorhabditis elegans, after exposure to copper nanoparticles with microbeam synchrotron radiation X-ray fluorescence".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 23.8(2008):1121-1124. |
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