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Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering
Hong XG(洪新国); Hong, XG; Weng, YX; Li, M
2004
发表期刊BIOPHYSICAL JOURNAL
卷号86期号:2页码:1082-1088
通讯作者Chinese Acad Sci, Inst Phys, Lab Soft Matter Phys, Beijing 100080, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing, Peoples R China
文章类型Article
摘要The topological shape of the integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria Rhodobacter spheroides 2.4.1 in detergent solution has been determined from synchrotron small-angle X-ray scattering data using direct curve-fitting by the ellipsoid, ab initio shape determination methods of simulated annealing algorithm and multipole expansion, respectively. The results indicate that the LH2 protein in aqueous solution is encapsulated by a monolayered detergent shell. The detergent-stabilized structure has the shape of an oblate plate, with a thickness of 40 Angstrom, a long axis of 110 Angstrom, and a short axis of 85 Angstrom. After correction for the detergent shell, the shape of the LH2 core is also an oblate plate with a height of 40 Angstrom, a long axis of 80 Angstrom, and a short axis of 55 Angstrom. In contrast to the cylindrical crystal structure with a height of 40 Angstrom and a diameter of 68 Angstrom, the molecular shape of the LH2 complex in detergent solution clearly deviates from the ringlike crystal structure, with an eccentricity found to be 0.59-consistent with the result of single molecular spectroscopy study of the isolated single LH2 molecules.
学科领域Biophysics
研究领域[WOS]Biophysics ; Biophysics
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语种英语
研究领域[WOS]Biophysics ; Biophysics
WOS类目Biophysics
WOS记录号WOS:000188437200038
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被引频次:27[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/239014
专题多学科研究中心
作者单位中国科学院高能物理研究所
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Hong XG,Hong, XG,Weng, YX,et al. Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering[J]. BIOPHYSICAL JOURNAL,2004,86(2):1082-1088.
APA 洪新国,Hong, XG,Weng, YX,&Li, M.(2004).Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering.BIOPHYSICAL JOURNAL,86(2),1082-1088.
MLA 洪新国,et al."Determination of the topological shape of integral membrane protein light-harvesting complex LH2 from photosynthetic bacteria in the detergent solution by small-angle X-ray scattering".BIOPHYSICAL JOURNAL 86.2(2004):1082-1088.
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