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In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy
Ye C(叶昶); Chen CY(陈春英); Chen Z(陈真); Meng H(孟幻); Li X(李湘); Yuan H(袁慧); Xing GM(邢更妹); Zhao F(赵峰); Zhao YL(赵宇亮); Chai ZF(柴之芳); Ye, C; Chen, CY; Chen, Z; Meng, H; Xing, L; Jiang, YX; Yuan, H; Xing, GM; Zhao, F; Zhao, YL; Chai, ZF; Fang, XH; Han, D; Chen, L; Wang, C; Wei, TT
刊名CHINESE SCIENCE BULLETIN
2006
卷号51期号:9页码:1060-1064
关键词fluorescence microscopy real time imaging fullerene derivative C-60(C(COOH)(2))(2) cross cellular membrane fluorescence labeling
学科分类Science & Technology - Other Topics
DOI10.1007/s11434-006-1060-1
通讯作者Chinese Acad Sci, Lab Bioenvironm Hlth Sci Nanoscale Mat, Inst High Energy Phys, Beijing 100049, Peoples R China ; Chinese Acad Sci, Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing 100080, Peoples R China ; Natl Ctr Nanosci & Technol, Beijing 100080, Peoples R China ; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
文章类型Article
英文摘要The interactions between nanoparticles and living cells were investigated by an imaging technique of fluorescence microscopy. For this purpose, the C-60 derivative C-60(C(COOH)(2))(2), a therapeutic agent for degeneration diseases of central nervous system, was synthesized, purified and characterized. Its interaction with the living cell and penetration of the cellular membrane were in situ studied using the real time imaging technique, and its potential cytotoxicity was also examined by flow cytometry. The results indicate that C-60(C(COOH)(2))(2) can easily enter cells, and is mainly located in cytoplasm by fluorescein labeling. Furthermore, C-60(C(COOH)(2))(2) can carry the molecule that cannot cross cellular membranes into cells, because fluorescein compound itself cannot enter the cell or adhere to membrane. At concentrations ranging from 1 x 10(-2) to 1 x 10(2) mg/L, C-60(C(COOH)(2))(2) does not show any detectable cytotoxicity.
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
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语种英语
WOS记录号WOS:000238882500007
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238976
专题院士_期刊论文
中国科学院高能物理研究所_加速器中心
中国科学院高能物理研究所_理论物理室
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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Ye C,Chen CY,Chen Z,et al. In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy[J]. CHINESE SCIENCE BULLETIN,2006,51(9):1060-1064.
APA 叶昶.,陈春英.,陈真.,孟幻.,李湘.,...&Wei, TT.(2006).In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy.CHINESE SCIENCE BULLETIN,51(9),1060-1064.
MLA 叶昶,et al."In situ observation of C-60(C(COOH)(2))(2) interacting with living cells using fluorescence microscopy".CHINESE SCIENCE BULLETIN 51.9(2006):1060-1064.
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