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Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom
Zhang, HM; Teng, M; Niu, LW; Wang, YB; Wang, YZ; Liu, Q; Huang, QQ; Hao, Q; Dong, YH; Liu, P; Dong YH(董宇辉); 刘鹏(多)
2004
Source PublicationACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Volume60Issue:5Pages:974-977
Corresponding AuthorChinese Acad Sci, Key Lab Struct Biol, Anhua 230026, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Anhua 230026, Peoples R China ; Cornell Univ, Cornell High Energy Synchrotron Source, MacCHESS, Ithaca, NY 14853 USA ; Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100039, Peoples R China
SubtypeArticle
AbstractA snake-venom protein named AHP-LAAO has been purified from Agkistrodon halys pallas venom using four-stage chromatography AHP-LAAO is a novel member of the snake-venom L-amino-acid oxidase family Its amino-acid sequence shows high homology to other members of this family. For L-leucine, the values of k(cat) and K-M are 31.1 s(-1) and 0.25 mM, respectively. The molecular weight of AHP-LAAO is about 60.7 kDa as determined by MALDI-TOF mass spectrometry. AHP-LAAO can also induce apoptosis of cultured Hela cells. Two sets of diffraction data with similar resolution limits (about 2.5 Angstrom) were collected independently at MacCHESS (Cornell High Energy Synchrotron Source, USA) and IHEP (Institute of High Energy Physics, Beijing, China). The crystals belong to space group I2(1)3, with unit-cell parameter a = 169.31 Angstrom, corresponding to one molecule in the asymmetric unit and a volume-to-weight ratio of 3.33 Angstrom(3) Da(-1). The final structural model is similar to that Of L-amino-acid oxidase from Calloselasma rhodostoma venom.
Subject AreaBiochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S0907444904000046
URL查看原文
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS SubjectBiochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS IDWOS:000220951000034
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Cited Times:51[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/238825
Collection多学科研究中心
实验物理中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Zhang, HM,Teng, M,Niu, LW,et al. Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2004,60(5):974-977.
APA Zhang, HM.,Teng, M.,Niu, LW.,Wang, YB.,Wang, YZ.,...&刘鹏.(2004).Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,60(5),974-977.
MLA Zhang, HM,et al."Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 60.5(2004):974-977.
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