Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom
Zhang, HM; Teng, M; Niu, LW; Wang, YB; Wang, YZ; Liu, Q; Huang, QQ; Hao, Q; Dong, YH; Liu, P; Dong YH(董宇辉); 刘鹏(多)
刊名ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
2004
卷号60期号:5页码:974-977
学科分类Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S0907444904000046
通讯作者Chinese Acad Sci, Key Lab Struct Biol, Anhua 230026, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Dept Mol & Cell Biol, Anhua 230026, Peoples R China ; Cornell Univ, Cornell High Energy Synchrotron Source, MacCHESS, Ithaca, NY 14853 USA ; Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100039, Peoples R China
文章类型Article
英文摘要A snake-venom protein named AHP-LAAO has been purified from Agkistrodon halys pallas venom using four-stage chromatography AHP-LAAO is a novel member of the snake-venom L-amino-acid oxidase family Its amino-acid sequence shows high homology to other members of this family. For L-leucine, the values of k(cat) and K-M are 31.1 s(-1) and 0.25 mM, respectively. The molecular weight of AHP-LAAO is about 60.7 kDa as determined by MALDI-TOF mass spectrometry. AHP-LAAO can also induce apoptosis of cultured Hela cells. Two sets of diffraction data with similar resolution limits (about 2.5 Angstrom) were collected independently at MacCHESS (Cornell High Energy Synchrotron Source, USA) and IHEP (Institute of High Energy Physics, Beijing, China). The crystals belong to space group I2(1)3, with unit-cell parameter a = 169.31 Angstrom, corresponding to one molecule in the asymmetric unit and a volume-to-weight ratio of 3.33 Angstrom(3) Da(-1). The final structural model is similar to that Of L-amino-acid oxidase from Calloselasma rhodostoma venom.
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Crystallography
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语种英语
WOS记录号WOS:000220951000034
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被引频次:43[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238825
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
作者单位中国科学院高能物理研究所
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Zhang, HM,Teng, M,Niu, LW,et al. Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2004,60(5):974-977.
APA Zhang, HM.,Teng, M.,Niu, LW.,Wang, YB.,Wang, YZ.,...&刘鹏.(2004).Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,60(5),974-977.
MLA Zhang, HM,et al."Purification, partial characterization, crystallization and structural determination of AHP-LAAO, a novel L-amino-acid oxidase with cell apoptosis-inducing activity from Agkistrodon halys pallas venom".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 60.5(2004):974-977.
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