SAD phasing by OASIS-2004: case studies of dual-space fragment extension
Yao DQ(姚德强); Huang S(黄胜); Yao, DQ; Huang, S; Wang, JW; Gu, YX; Zheng, CD; Fan, HF; Watanabe, N; Tanaka, I
刊名ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
2006
卷号62期号:8页码:883-890
学科分类Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S0907444906006445
通讯作者Chinese Acad Sci, Inst Phys, Beijing Natl Lab Condensed Matter Phys, Beijing 100080, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Anhua 230026, Peoples R China ; Hokkaido Univ, Grad Sch Sci, Sapporo, Hokkaido 0600810, Japan
文章类型Article
英文摘要The principle of dual- space phasing is used in dealing with protein SAD data. Four programs are involved in iterative dual- space fragment extension to improve automatic model building. OASIS- 2004 is used to break the phase ambiguity intrinsic in the SAD experiment. In the initial cycle, discrimination of SAD phase doublets is performed by the direct method incorporating the known anomalous- scattering substructure. In subsequent cycles, discrimination is performed by the direct method incorporating both the known anomalous- scattering substructure and the partial protein structure obtained from model building in the preceding cycle. DM is used to improve direct- method phases via density modification. RESOLVE is used for initial model building and ARP/ wARP is used to complete the structure. Case studies with three sets of difficult SAD data showed that the procedure is beneficial to high- throughput proteinstructure determination and all of the four programs involved make their unique contribution to the process.
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Crystallography
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语种英语
WOS记录号WOS:000239119800006
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238678
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
作者单位中国科学院高能物理研究所
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Yao DQ,Huang S,Yao, DQ,et al. SAD phasing by OASIS-2004: case studies of dual-space fragment extension[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2006,62(8):883-890.
APA 姚德强.,黄胜.,Yao, DQ.,Huang, S.,Wang, JW.,...&Tanaka, I.(2006).SAD phasing by OASIS-2004: case studies of dual-space fragment extension.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,62(8),883-890.
MLA 姚德强,et al."SAD phasing by OASIS-2004: case studies of dual-space fragment extension".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 62.8(2006):883-890.
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