Determination of copper binding in Pseudomonas putida CZ1 by chemical modifications and X-ray absorption spectroscopy
Chen, XC; Shi, JY; Chen, YX; Xu, XJ; Chen, LT; Wang, H; Hu, TD; Hu TD(胡天斗)
刊名APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
2007
卷号74期号:4页码:881-889
关键词Pseudomonas putida CZ1 chemical modification FTIR XAS metal binding
学科分类Biotechnology & Applied Microbiology
DOI10.1007/s00253-006-0592-2
通讯作者Zhejiang Univ, Dept Environm Engn, Hangzhou 310029, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China
文章类型Article
英文摘要Previously performed studies have shown that Pseudomonas putida CZ1 biomass can bind an appreciable amount of Cu(II) and Zn(II) ions from aqueous solutions. The mechanisms of Cu- and Zn-binding by P. putida CZ1 were ascertained by chemical modifications of the biomass followed by Fourier transform infrared and X-ray absorption spectroscopic analyses of the living or nonliving cells. A dramatic decrease in Cu(II)- and Zn(II)-binding resulted after acidic methanol esterification of the nonliving cells, indicating that carboxyl functional groups play an important role in the binding of metal to the biomaterial. X-ray absorption spectroscopy was used to determine the speciation of Cu ions bound by living and nonliving cells, as well as to elucidate which functional groups were involved in binding of the Cu ions. The X-ray absorption near-edge structure spectra analysis showed that the majority of the Cu was bound in both samples as Cu(II). The fitting results of Cu K-edge extended X-ray absorption fine structure spectra showed that N/O ligands dominated in living and nonliving cells. Therefore, by combining different techniques, our results indicate that carboxyl functional groups are the major ligands responsible for the metal binding in P. putida CZ1.
类目[WOS]Biotechnology & Applied Microbiology
研究领域[WOS]Biotechnology & Applied Microbiology
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语种英语
WOS记录号WOS:000244451200020
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238673
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
作者单位中国科学院高能物理研究所
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Chen, XC,Shi, JY,Chen, YX,et al. Determination of copper binding in Pseudomonas putida CZ1 by chemical modifications and X-ray absorption spectroscopy[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2007,74(4):881-889.
APA Chen, XC.,Shi, JY.,Chen, YX.,Xu, XJ.,Chen, LT.,...&胡天斗.(2007).Determination of copper binding in Pseudomonas putida CZ1 by chemical modifications and X-ray absorption spectroscopy.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,74(4),881-889.
MLA Chen, XC,et al."Determination of copper binding in Pseudomonas putida CZ1 by chemical modifications and X-ray absorption spectroscopy".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 74.4(2007):881-889.
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