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A large-scale, high-efficiency and low-cost platform for structural genomics studies
Su, XD; Liang, YH; Li, LF; Nan, J; Brostromer, E; Liu, P; Dong, YH; Xian, DC; Liu P(刘鹏); Dong YH(董宇辉); Xian DC(冼鼎昌)
2006
发表期刊ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
卷号62期号:8页码:843-851
通讯作者Peking Univ, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China
摘要A large- scale, high- efficiency and low- cost platform based on a Beckman Coulter Biomek FX and custom- made automation systems for structural genomics has been set up at Peking University, Beijing, People's Republic of China. This platform has the capacity to process up to 2000 genes per year for structural and functional analyses. Bacillus subtilis, a model organism for Gram- positive bacteria, and Streptococcus mutans, a major pathogen of dental caries, were selected as the main targets. To date, more than 470 B. subtilis and 1200 S. mutans proteins and hundreds of proteins from other sources, including human liver proteins, have been selected as targets for this platform. The selected genes are mainly related to important metabolism pathways and/ or have potential relevance for drug design. To date, 40 independent structures have been determined; of these 11 are in the category of novel structures by the criterion of having less than 30% sequence identity to known structures. More than 13 structures were determined by SAD/ MAD phasing. The macromolecular crystallography beamline at the Beijing Synchrotron Radiation Facility and modern phasing programs have been crucial components of the operation of the platform. The idea and practice of the genomic approach have been successfully adopted in a moderately funded structural biology program and it is believed this adaptation will greatly improve the production of protein structures. The goal is to be able to solve a protein structure of moderate difficulty at a cost about US $ 10 000.
文章类型Article
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S0907444906024395
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics ; Crystallography
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语种英语
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS记录号WOS:000239119800001
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被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238594
专题院士
作者单位中国科学院高能物理研究所
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Su, XD,Liang, YH,Li, LF,et al. A large-scale, high-efficiency and low-cost platform for structural genomics studies[J]. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,2006,62(8):843-851.
APA Su, XD.,Liang, YH.,Li, LF.,Nan, J.,Brostromer, E.,...&冼鼎昌.(2006).A large-scale, high-efficiency and low-cost platform for structural genomics studies.ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY,62(8),843-851.
MLA Su, XD,et al."A large-scale, high-efficiency and low-cost platform for structural genomics studies".ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY 62.8(2006):843-851.
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