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Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry
Yu HW(喻宏伟); Chen CY(陈春英); Gao YX(高愈希); Li B(李柏); Chai ZF(柴之芳); Yu, HW; Chen, CY; Gao, YX; Li, B; Chai, ZF
刊名CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2006
卷号34期号:6页码:749-753
关键词reversed ion-pair high performance liquid chromatography inductively coupled plasma mass spectrometry hyphenated technique biological samples speciation analysis selenium
学科分类Chemistry
DOI10.1016/S1872-2040(06)60036-4
通讯作者Chinese Acad Sci, Key Lab Nucl Analyt Tech, Inst High Energy Phys, Beijing 100049, Peoples R China
文章类型Article
英文摘要A hyphenated technique for chemical speciation analysis of selenium in biological samples was described. A reversed ion-pair high performance liquid chromatograph is introduced for separating selenocompounds, at the meantime an inductively coupled plasma mass spectrometry was combined for on-line elemental determination. A series of standard compounds were used to achieve qualitative and quantitative analysis. Seven seleno-containing compounds, Na2SeO4, Na2SeO3, trimethyl selenonium iodide ( TmSeI ), selenocystaine ( SeCys ), selenourea ( SeUr ), selenomethionine ( SeMet ) and selenocystamine ( SeCM ), can be individually detected. The detection limits are 0.1-1.5 mu g/L, and the linear calibration range for Se concentrations is between 1 and 100 mu g/L. The method was also applied to analyze human liver cytosolic extracts, human serum, and human urine. Only 50 mu L of the samples are needed and the recoveries of spiked seleno-compounds are 93% -117%. The hyphenated method possesses the merits of simple operation, high sensitivity and good repeatability, which is a feasible method for speciation analysis of small molecular selenocompounds in different biological samples.
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
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语种英语
WOS记录号WOS:000240869900002
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文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238420
专题院士_期刊论文
中国科学院高能物理研究所_实验物理中心
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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Yu HW,Chen CY,Gao YX,et al. Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2006,34(6):749-753.
APA 喻宏伟.,陈春英.,高愈希.,李柏.,柴之芳.,...&Chai, ZF.(2006).Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,34(6),749-753.
MLA 喻宏伟,et al."Chemical speciation analysis of selenium in biological samples by a hyphenated technique of high performance liquid chromatography-inductively coupled plasma mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 34.6(2006):749-753.
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