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ATP-induced noncooperative thermal unfolding of hen lysozyme
Liu, HL; Yin, PD; He, SN; Sun, ZH; Tao, Y; Huang, Y; Zhuang, H; Zhang, GB; Wei, SQ; Tao Y(陶冶); Huang Y(黄艳); Zhuang H(庄浩)
Corresponding Author[Liu, Honglin ; Yin, Peidong ; He, Shengnan ; Sun, Zhihu ; Zhang, Guobin ; Wei, Shiqiang] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China ; [Tao, Ye ; Huang, Yan ; Zhuang, Hao] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China
AbstractTo understand the role of ATP underlying the enhanced amyloidosis of hen egg white lysozyme (HEWL), the synchrotron radiation circular dichroism, combined with tryptophan fluorescence, dynamic light-scattering, and differential scanning calorimetry, is used to examine the alterations of the conformation and thermal unfolding pathway of the HEWL in the presence of ATP, Mg(2+)-ATP, ADP, AMP, etc. It is revealed that the binding of ATP to HEWL through strong electrostatic interaction changes the secondary structures of HEWL and makes the exposed residue W62 move into hydrophobic environments. This alteration of W62 decreases the beta-domain stability of HEWL, induces a noncooperative unfolding of the secondary structures, and produces a partially unfolded intermediate. This intermediate containing relatively rich alpha-helix and less beta-sheet structures has a great tendency to aggregate. The results imply that the ease of aggregating of HEWL is related to the extent of denaturation of the amyloidogenic region, rather than the electrostatic neutralizing effect or monomeric beta-sheet enriched intermediate. (C) 2010 Elsevier Inc. All rights reserved.
KeywordHen egg white lysozyme Synchrotron radiation circular dichroism Secondary structure Noncooperative unfolding Partially unfolded intermediate
Subject AreaBiochemistry & Molecular Biology; Biophysics
Indexed BySCI
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000279974600041
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Document Type期刊论文
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Liu, HL,Yin, PD,He, SN,et al. ATP-induced noncooperative thermal unfolding of hen lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,397(3):598-602.
APA Liu, HL.,Yin, PD.,He, SN.,Sun, ZH.,Tao, Y.,...&庄浩.(2010).ATP-induced noncooperative thermal unfolding of hen lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,397(3),598-602.
MLA Liu, HL,et al."ATP-induced noncooperative thermal unfolding of hen lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 397.3(2010):598-602.
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