ATP-induced noncooperative thermal unfolding of hen lysozyme
Liu, HL; Yin, PD; He, SN; Sun, ZH; Tao, Y; Huang, Y; Zhuang, H; Zhang, GB; Wei, SQ; Tao Y(陶冶); Huang Y(黄艳); Zhuang H(庄浩)
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2010
卷号397期号:3页码:598-602
关键词Hen egg white lysozyme Synchrotron radiation circular dichroism Secondary structure Noncooperative unfolding Partially unfolded intermediate
学科分类Biochemistry & Molecular Biology; Biophysics
DOI10.1016/j.bbrc.2010.06.005
通讯作者[Liu, Honglin ; Yin, Peidong ; He, Shengnan ; Sun, Zhihu ; Zhang, Guobin ; Wei, Shiqiang] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230029, Anhui, Peoples R China ; [Tao, Ye ; Huang, Yan ; Zhuang, Hao] Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China
文章类型Article
英文摘要To understand the role of ATP underlying the enhanced amyloidosis of hen egg white lysozyme (HEWL), the synchrotron radiation circular dichroism, combined with tryptophan fluorescence, dynamic light-scattering, and differential scanning calorimetry, is used to examine the alterations of the conformation and thermal unfolding pathway of the HEWL in the presence of ATP, Mg(2+)-ATP, ADP, AMP, etc. It is revealed that the binding of ATP to HEWL through strong electrostatic interaction changes the secondary structures of HEWL and makes the exposed residue W62 move into hydrophobic environments. This alteration of W62 decreases the beta-domain stability of HEWL, induces a noncooperative unfolding of the secondary structures, and produces a partially unfolded intermediate. This intermediate containing relatively rich alpha-helix and less beta-sheet structures has a great tendency to aggregate. The results imply that the ease of aggregating of HEWL is related to the extent of denaturation of the amyloidogenic region, rather than the electrostatic neutralizing effect or monomeric beta-sheet enriched intermediate. (C) 2010 Elsevier Inc. All rights reserved.
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
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语种英语
WOS记录号WOS:000279974600041
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/238071
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_计算中心
中国科学院高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
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Liu, HL,Yin, PD,He, SN,et al. ATP-induced noncooperative thermal unfolding of hen lysozyme[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2010,397(3):598-602.
APA Liu, HL.,Yin, PD.,He, SN.,Sun, ZH.,Tao, Y.,...&庄浩.(2010).ATP-induced noncooperative thermal unfolding of hen lysozyme.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,397(3),598-602.
MLA Liu, HL,et al."ATP-induced noncooperative thermal unfolding of hen lysozyme".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 397.3(2010):598-602.
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