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Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens
Zhou, DW; Sun, JP; Zhao, W; Zhang, X; Shi, YY; Teng, MK; Niu, LW; Dong, YH; Dong YH(董宇辉); 刘鹏(多)
2006
发表期刊ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
卷号62期号:5页码:474-476
通讯作者Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China ; Chinese Acad Sci, Key Lab Struct Biol, Hefei 230027, Anhui, Peoples R China ; Chinese Acad Sci, Beijing Synchrotron Radiat Facil, Inst High Energy Phys, Beijing 100039, Peoples R China
文章类型Article
摘要Galectins are a family of animal lectins which share similar carbo-hydraterecognition domains (CRDs) and an affinity for beta-galactosides. A novel human galectin-related protein named GRP (galectin-related protein; previously known as HSPC159) comprises only one conserved CRD with 38 additional N-terminal residues. The C-terminal fragment of human GRP (GRP-C; residues 38-172) containing the CRD has been expressed and purified. The protein was crystallized using the hanging-drop vapour-diffusion method from a solution containing 2% PEG 400 and 2M ammonium sulfate in 100 mM Tris-HCl buffer pH 7.5. Diffraction data were collected to a resolution limit of 2.0 angstrom at beamline 3W1A of Beijing Synchrotron Radiation Facility at 100 K. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 123.07, b = 96.67, c = 61.56 angstrom, beta = 118.72 degrees. The estimated Matthews coefficient was 2.6 angstrom(3) Da(-1), corresponding to 51.8% solvent content.
学科领域Biochemistry & Molecular Biology; Biophysics; Crystallography
DOI10.1107/S1744309106012875
URL查看原文
语种英语
WOS研究方向Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS类目Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
WOS记录号WOS:000237159000015
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237636
专题多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Zhou, DW,Sun, JP,Zhao, W,et al. Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2006,62(5):474-476.
APA Zhou, DW.,Sun, JP.,Zhao, W.,Zhang, X.,Shi, YY.,...&刘鹏.(2006).Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,62(5),474-476.
MLA Zhou, DW,et al."Expression, purification, crystallization and preliminary X-ray characterization of the GRP carbohydrate-recognition domain from Homo sapiens".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 62.5(2006):474-476.
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