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Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues
Gao YX(高愈希); Liu yingbin; Chen CY(陈春英); Li B(李柏); He W(何伟); Huang YY(黄宇营); Chai ZF(柴之芳); Gao, YX; Liu, YB; Chen, CY; Li, B; He, W; Huang, YY; Chai, ZF
2005
发表期刊JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷号20期号:5页码:473-475
通讯作者Chinese Acad Sci, Inst High Energy Phys, Beijing 100039, Peoples R China ; Zhejiang Univ, Affiliated Hosp 2, Dept Surg, Hangzhou 310009, Peoples R China
摘要The combined use of synchrotron radiation X-ray fluorescence (SRXRF) and isoelectric focusing (IEF) has been developed that allows comparison of the distribution of metal-containing proteins in cytosol of human hepatocellular carcinoma (HCC) with its surrounding "normal'' tissues. Proteins in samples from two individuals were separated with thin layer IEF, and the contents of Fe, Cu and Zn in protein bands were determined with SRXRF after drying the IEF gel. The results show that the metal-containing proteins in these samples of hepatocellular cytosol are specific for a given patient, and the distribution patterns of metal-containing proteins are distinguishable between the HCC and "normal'' tissues. The technique has the advantages of quantifying trace elements and identifying proteins simultaneously.
文章类型Article
学科领域Chemistry; Spectroscopy
DOI10.1039/b413309a
研究领域[WOS]Chemistry ; Spectroscopy
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语种英语
WOS类目Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000228689500015
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被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237630
专题院士
实验物理中心
多学科研究中心
作者单位中国科学院高能物理研究所
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GB/T 7714
Gao YX,Liu yingbin,Chen CY,et al. Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2005,20(5):473-475.
APA 高愈希.,Liu yingbin.,陈春英.,李柏.,何伟.,...&Chai, ZF.(2005).Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,20(5),473-475.
MLA 高愈希,et al."Combination of synchrotron radiation X-ray fluorescence with isoelectric focusing for study of metalloprotein distribution in cytosol of hepatocellular carcinoma and surrounding normal tissues".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 20.5(2005):473-475.
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