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Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy
Zhao, W; Chu WS(储旺盛); Yang FF(杨飞飞); Yu MJ(于梅娟); Chen DL(陈栋梁); Guo XY(郭兴运); Zhou DW(周大卫); Wu ZY(吴自玉); Chu, WS; Yang, FF; Yub, MJ; Chen, DL; Guo, XY; Zhou, DW; Shi, N; Marcelli, A; Niu, LW; Teng, MK; Gong, WM; Benfatto, M; Wu, ZY
2007
发表期刊NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
卷号580期号:1页码:451-456
通讯作者Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Beijing 100049, Peoples R China ; Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230027, Anhui, Peoples R China ; Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Anhui, Peoples R China ; Ist Nazl Fis Nucl, Lab Nazl Frascati, I-00044 Frascati, Italy ; Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China
文章类型Article; Proceedings Paper
摘要The last several years have witnessed a tremendous increase in biological applications using X-ray absorption spectroscopy (BioXAS), thanks to continuous advancements in synchrotron radiation (SR) sources and detector technology. However, XAS applications in many biological systems have been limited by the intrinsic limitations of the Extended X-ray Absorption Fine Structure (EXAFS) technique e.g., the lack of sensitivity to bond angles. As a consequence, the application of the X-ray absorption near-edge structure (XANES) spectroscopy changed this scenario that is now continuously changing with the introduction of the first quantitative XANES packages such as Minut XANES (MXAN). Here we present and discuss the XANES code MXAN, a novel XANES-fitting package that allows a quantitative analysis of experimental data applied to Zn K-edge spectra of two metalloproteins: Leptospira interrogans Peptide deformylase (LiPDF) and acutolysin-C, a representative of snake venom metalloproteinases (SVMPs) from Agkistrodon acutus venom. The analysis on these two metallohydrolases reveals that proteolytic activities are correlated to subtle conformation changes around the zinc ion. In particular, this quantitative study clarifies the occurrence of the LiPDF catalytic mechanism via a two-water-molecules model, whereas in the acutolysin-C we have observed a different proteolytic activity correlated to structural changes around the zinc ion induced by pH variations. (C) 2007 Elsevier B.V. All rights reserved.
关键词BioXAS MXAN XANES metalloprotein LiPDF acutolysiu-C
学科领域Instruments & Instrumentation; Nuclear Science & Technology; Physics; Spectroscopy
研究领域[WOS]Instruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy ; Instruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy
DOI10.1016/j.nima.2007.05.196
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语种英语
研究领域[WOS]Instruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy ; Instruments & Instrumentation ; Nuclear Science & Technology ; Physics ; Spectroscopy
WOS类目Instruments & Instrumentation ; Nuclear Science & Technology ; Physics, Particles & Fields ; Spectroscopy
WOS记录号WOS:000249741300117
引用统计
被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237534
专题多学科研究中心
实验物理中心
粒子天体物理中心
作者单位中国科学院高能物理研究所
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Zhao, W,Chu WS,Yang FF,et al. Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy[J]. NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,2007,580(1):451-456.
APA Zhao, W.,储旺盛.,杨飞飞.,于梅娟.,陈栋梁.,...&Wu, ZY.(2007).Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy.NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT,580(1),451-456.
MLA Zhao, W,et al."Quantitative investigation of two metallohydrolases by X-ray absorption spectroscopy near-edge spectroscopy".NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT 580.1(2007):451-456.
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