IHEP OpenIR  > 院士
Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection
Chen CY(陈春英); Zhao JJ(赵九江); Zhang PQ(章佩群); Chai ZF(柴之芳); Chen, CY; Zhao, JJ; Zhang, PQ; Chai, ZF
2002
发表期刊ANALYTICAL AND BIOANALYTICAL CHEMISTRY
卷号372期号:3页码:426-430
通讯作者Acad Sinica, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China
文章类型Article; Proceedings Paper
摘要Speciation of Se-containing proteins in the subcellular fractions of human liver was studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by hydride generation-atomic fluorescence spectrometric (HG-AFS) detection. It was found that about 24 kinds of Se-containing proteins existed in subcellular fractions of normal human liver. The molecular weights (MW) of the subunits were mostly in the range 20-30 kDa and 50-80 kDa. Major Se-containing protein fractions at 61 kDa and 21 kDa are probably selenoprotein P and glutathione peroxidase, respectively. The 54 kDa protein is probably a thioredoxin reductase, which is presented in nuclei, mitochondria, lysosome, microsome and cytosol. We noticed that the Se-containing protein with the lowest MW of 9.3 kDa only existed in lysosome. Most of the proteins have not been identified and would require further investigation to characterize them. The specific subcellular distributions of different Se-containing proteins suggest that they could play important biological roles in each organelle.
关键词Se-containing proteins gel electrophoresis speciation HG-AFS human liver
学科领域Biochemistry & Molecular Biology; Chemistry
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Biochemistry & Molecular Biology ; Chemistry
DOI10.1007/s00216-001-1212-8
URL查看原文
语种英语
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Biochemistry & Molecular Biology ; Chemistry
WOS类目Biochemical Research Methods ; Chemistry, Analytical
WOS记录号WOS:000176184900006
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237434
专题院士
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Chen CY,Zhao JJ,Zhang PQ,et al. Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2002,372(3):426-430.
APA 陈春英.,赵九江.,章佩群.,柴之芳.,Chen, CY.,...&Chai, ZF.(2002).Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,372(3),426-430.
MLA 陈春英,et al."Speciation and subcellular location of Se-containing proteins in human liver studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and hydride generation-atomic fluorescence spectrometric detection".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 372.3(2002):426-430.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
3932.pdf(69KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[陈春英]的文章
[赵九江]的文章
[章佩群]的文章
百度学术
百度学术中相似的文章
[陈春英]的文章
[赵九江]的文章
[章佩群]的文章
必应学术
必应学术中相似的文章
[陈春英]的文章
[赵九江]的文章
[章佩群]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。