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Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines
Miles, AJ; Janes, RW; Brown, A; Clarke, DT; Sutherland, JC; Tao, Y; Wallace, BA; Hoffmann, SV; Tao Y(陶冶)
2008
Source PublicationJOURNAL OF SYNCHROTRON RADIATION
Volume15Issue:4Pages:420-422
Corresponding Author[Hoffmann, S. V.] Univ Aarhus, Inst Storage Ring Facil, DK-8000 Aarhus C, Denmark ; [Miles, A. J. ; Wallace, B. A.] Univ London, Birkbeck Coll, Dept Crystallog, London WC1E 7HX, England ; [Janes, Robert W.] Univ London, Sch Biol & Chem Sci, London E1 4NS, England ; [Brown, A. ; Clarke, D. T.] STFC, Daresbury Lab, Warrington WA4 4AD, Cheshire, England ; [Sutherland, J. C.] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA ; [Sutherland, J. C.] E Carolina Univ, Dept Phys, Greenville, NC 27858 USA ; [Tao, Y.] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing, Peoples R China
SubtypeArticle
AbstractNew high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
Keywordprotein denaturation radiation damage synchrotron radiation circular dichroism spectroscopy photon flux vacuum ultraviolet wavelengths
Subject AreaInstruments & Instrumentation; Optics; Physics
DOI10.1107/S0909049508009606
URL查看原文
Language英语
WOS Research AreaInstruments & Instrumentation ; Optics ; Physics
WOS SubjectInstruments & Instrumentation ; Optics ; Physics, Applied
WOS IDWOS:000256720900016
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Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/237408
Collection多学科研究中心
Affiliation中国科学院高能物理研究所
First Author AffilicationInstitute of High Energy
Recommended Citation
GB/T 7714
Miles, AJ,Janes, RW,Brown, A,et al. Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines[J]. JOURNAL OF SYNCHROTRON RADIATION,2008,15(4):420-422.
APA Miles, AJ.,Janes, RW.,Brown, A.,Clarke, DT.,Sutherland, JC.,...&陶冶.(2008).Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines.JOURNAL OF SYNCHROTRON RADIATION,15(4),420-422.
MLA Miles, AJ,et al."Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines".JOURNAL OF SYNCHROTRON RADIATION 15.4(2008):420-422.
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