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Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines
Miles, AJ; Janes, RW; Brown, A; Clarke, DT; Sutherland, JC; Tao, Y; Wallace, BA; Hoffmann, SV; Tao Y(陶冶)
2008
发表期刊JOURNAL OF SYNCHROTRON RADIATION
卷号15期号:4页码:420-422
通讯作者[Hoffmann, S. V.] Univ Aarhus, Inst Storage Ring Facil, DK-8000 Aarhus C, Denmark ; [Miles, A. J. ; Wallace, B. A.] Univ London, Birkbeck Coll, Dept Crystallog, London WC1E 7HX, England ; [Janes, Robert W.] Univ London, Sch Biol & Chem Sci, London E1 4NS, England ; [Brown, A. ; Clarke, D. T.] STFC, Daresbury Lab, Warrington WA4 4AD, Cheshire, England ; [Sutherland, J. C.] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA ; [Sutherland, J. C.] E Carolina Univ, Dept Phys, Greenville, NC 27858 USA ; [Tao, Y.] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing, Peoples R China
文章类型Article
摘要New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
关键词protein denaturation radiation damage synchrotron radiation circular dichroism spectroscopy photon flux vacuum ultraviolet wavelengths
学科领域Instruments & Instrumentation; Optics; Physics
研究领域[WOS]Instruments & Instrumentation ; Optics ; Physics ; Instruments & Instrumentation ; Optics ; Physics
DOI10.1107/S0909049508009606
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语种英语
研究领域[WOS]Instruments & Instrumentation ; Optics ; Physics ; Instruments & Instrumentation ; Optics ; Physics
WOS类目Instruments & Instrumentation ; Optics ; Physics, Applied
WOS记录号WOS:000256720900016
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被引频次:55[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237408
专题多学科研究中心
作者单位中国科学院高能物理研究所
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Miles, AJ,Janes, RW,Brown, A,et al. Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines[J]. JOURNAL OF SYNCHROTRON RADIATION,2008,15(4):420-422.
APA Miles, AJ.,Janes, RW.,Brown, A.,Clarke, DT.,Sutherland, JC.,...&陶冶.(2008).Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines.JOURNAL OF SYNCHROTRON RADIATION,15(4),420-422.
MLA Miles, AJ,et al."Light flux density threshold at which protein denaturation is induced by synchrotron radiation circular dichroism beamlines".JOURNAL OF SYNCHROTRON RADIATION 15.4(2008):420-422.
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