IHEP OpenIR  > 院士
An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence
其他题名一种改进的同步辐射X射线荧光原位分析人肝细胞胞质溶胶内金属蛋白分布的方法
Gao YX(高愈希); Chen CY(陈春英); Zhao JJ(赵九江); Zhang PQ(章佩群); Chai ZF(柴之芳); He W(何伟); Huang YY(黄宇营); Gao, YX; Chen, CY; Zhao, JJ; Zhang, PQ; Chai, ZF; He, W; Huang, YY
2003
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号31期号:4页码:395-398
通讯作者Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China ; Chinese Acad Sci, Inst High Energy Phys, Synchrotron Radiat Lab, Beijing 100039, Peoples R China
文章类型Article
摘要A method for the analysis of trace elements in protein bands with synchrotron radiation X-ray fluorescence (SRXFR) after electrophoresis separation was improved. Through a step of drying the gel before determination, the continues background mainly originated from the Compton scattering of X-ray upon the dry gel matrix was reduced to about one tenth of that upon the wet gel. Trace elements lighter than zinc in protein bands can be determined simultaneously. The method could be used for speciation of trace elements in biological sample.. Applying the improved procedure to investigate the distribution of metalloproteins in human liver cytosol,. six Zn-containing proteins with molecular weights(MWs) of 17.5, 20.5, 27, 35, 55, and 6 3 kdalton respectively, and four Fe-containing proteins with MWs of 20, 23, 43, and 83.5 kdalton respectively can be detected.. The 63 kdalton Zn-containing protein is the dominant form of zinc in the sample.
关键词synchrotron radiation X-ray fluorescence analysis metalloprotein electrophoretic separation
学科领域Chemistry
研究领域[WOS]Chemistry ; Chemistry
URL查看原文
语种英语
研究领域[WOS]Chemistry ; Chemistry
WOS类目Chemistry, Analytical
WOS记录号WOS:000182746400003
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237306
专题院士
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Gao YX,Chen CY,Zhao JJ,et al. An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2003,31(4):395-398.
APA 高愈希.,陈春英.,赵九江.,章佩群.,柴之芳.,...&Huang, YY.(2003).An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,31(4),395-398.
MLA 高愈希,et al."An improved method for analysis of distribution of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 31.4(2003):395-398.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
3712.pdf(200KB)期刊论文作者接受稿限制开放CC BY-NC-SA请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[赵九江]的文章
百度学术
百度学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[赵九江]的文章
必应学术
必应学术中相似的文章
[高愈希]的文章
[陈春英]的文章
[赵九江]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。