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Study of chromium-containing proteins in subcellular fractions of rat liver by enriched stable isotopic tracer technique and gel filtration chromatography
Feng WY(丰伟悦); Li B(李柏); Liu J(刘景); Chai ZF(柴之芳); Zhang PQ(章佩群); Gao YX(高义贤); Zhao JJ(赵九江); Feng, WY; Li, B; Liu, J; Chai, ZF; Zhang, PQ; Gao, YX; Zhao, JJ
刊名ANALYTICAL AND BIOANALYTICAL CHEMISTRY
2003
卷号375期号:5页码:363-368
关键词Cr-containing protein speciation enriched stable isotopic tracer technique subcellular rat
学科分类Biochemistry & Molecular Biology; Chemistry
DOI10.1007/s00216-002-1691-2
通讯作者Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, Beijing 100039, Peoples R China
文章类型Article
英文摘要Ten male Wistar rats were intravenously injected with a single approximately physiological dose of enriched stable isotopic Cr-50 tracer solution (200ng Cr-50(3+)/100 g body wt). The fundamental distribution patterns of the chromium-containing proteins in the nucleic, mitochondrial, lysosomal, microsomal, and cytosolic subcellular fractions of the rat liver were investigated by means of Sephadex. G-100 gel chromatography combined with neutron activation analysis via Cr-50 (n, gamma) Cr-51 reaction. In total, nine kinds of Cr-containing proteins were found in the five subcellular fractions, whose relative molecular masses were 96.6+/-6.2, 68.2+/-1.4, 57.9+/-4.7, 36.6+/-1.2, 24.2+/-1.8, 14.0+/-1.5, 8.8+/-0.6, 6.9+/-0.4, and 4.2+/-0.4 kDa. Approximately 64.5% of Cr proteins accumulated in the cytosolic fraction. The second enriched part was the nucleic fraction; about 12.2% Cr proteins were stored in this section. The 4.2-kDa molecular mass might contain the so-called low molecular weight chromium-containing substance; however, in this research, it was only observed in the mitochondria, lysosome, and microsome. In the mitochondrial fraction, most of the Cr proteins were present as relatively low molecular weight substances: about 56% of chromium-containing proteins had molecular masses less than or equal to6.9 kDa. Nevertheless, more than 69% of Cr-containing proteins were observed with molecular masses greater than or equal to57.9 kDa in the liver cytosolic fraction.
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
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语种英语
WOS记录号WOS:000181389900006
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被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237168
专题院士_期刊论文
中国科学院高能物理研究所_实验物理中心
中国科学院高能物理研究所_多学科研究中心
中国科学院高能物理研究所_中国散裂中子源
作者单位中国科学院高能物理研究所
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Feng WY,Li B,Liu J,et al. Study of chromium-containing proteins in subcellular fractions of rat liver by enriched stable isotopic tracer technique and gel filtration chromatography[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2003,375(5):363-368.
APA 丰伟悦.,李柏.,刘景.,柴之芳.,章佩群.,...&Zhao, JJ.(2003).Study of chromium-containing proteins in subcellular fractions of rat liver by enriched stable isotopic tracer technique and gel filtration chromatography.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,375(5),363-368.
MLA 丰伟悦,et al."Study of chromium-containing proteins in subcellular fractions of rat liver by enriched stable isotopic tracer technique and gel filtration chromatography".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 375.5(2003):363-368.
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