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Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes
Li YH(李艳华); Gao ZQ(高增强); Hou HF(侯海峰); Dong YH(董宇辉); Li, YH; Gao, ZQ; Hou, HF; Li, L; Zhang, JH; Yang, HH; Dong, YH; Tan, HR
2011
发表期刊BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷号405期号:3页码:344-348
通讯作者[Li, Yanhua ; Gao, Zengqiang ; Hou, Haifeng ; Dong, Yuhui] Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China ; [Li, Yanhua ; Li, Lei ; Zhang, Jihui ; Yang, Haihua ; Tan, Huarong] Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China
摘要Nitroalkane oxidase (NAO) catalyzes neutral nitroalkanes to their corresponding aldehydes or ketones, hydrogen peroxide and nitrite. The crystal structure of NAO from Streptomyces ansochromogenes was determined; it consists of two domains, a TIM barrel domain bound to FMN and C-terminal domain with a novel folding pattern. Site-directed mutagenesis of His(179), which is spatially adjacent to FMN, resulted in the loss of enzyme activity, demonstrating that this amino acid residue is important for catalysis. The crystal structure of mutant H179D-nitroethane was also analyzed. Interestingly, Sa-NAO shows the typical function as nitroalkane oxidase but its structure is similar to that of 2-nitropropane dioxygenase. Overall, these results suggest that Sa-NAO is a novel nitroalkane oxidase with TIM barrel structure. (C) 2010 Elsevier Inc. All rights reserved.
文章类型Article
关键词Nitroalkane oxidase Crystallization Site-directed mutagenesis TIM barrel
学科领域Biochemistry & Molecular Biology; Biophysics
DOI10.1016/j.bbrc.2010.12.050
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
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语种英语
WOS类目Biochemistry & Molecular Biology ; Biophysics
WOS记录号WOS:000287996500003
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/237073
专题多学科研究中心
粒子天体物理中心
作者单位中国科学院高能物理研究所
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Li YH,Gao ZQ,Hou HF,et al. Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2011,405(3):344-348.
APA 李艳华.,高增强.,侯海峰.,董宇辉.,Li, YH.,...&Tan, HR.(2011).Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,405(3),344-348.
MLA 李艳华,et al."Crystal structure and site-directed mutagenesis of a nitroalkane oxidase from Streptomyces ansochromogenes".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 405.3(2011):344-348.
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