; Ze, Yuguang
; Liu, Jie
; Liu, Huiting
; Liu, Chao
; Yan, Jinying
; Duan, Yanmei
; Hong, Fashui] Soochow Univ, Coll Med, Suzhou 215123, Peoples R China
; [Li, Zhongrui
; Zhao, Jinfang] Univ Arkansas, Nanotechnol Ctr, Little Rock, AR 72204 USA
; [Xie, Yaning] Chinese Acad Sci, Inst High Energy Phys, Synchrotron Radiat Lab, Beijing 100039, Peoples R China
Nano-TiO(2) and Superoxide dismutase (SOD, EC 188.8.131.52) have been added to cosmetics and used to prevent injury of skin from UV-radiation, which might be rela red to the decrease of oxidative damage of skin. In previous studies we had proven that nano-anatase could increase the activity of SOD and decrease the oxidative damage in vivo. The mechanisms by which nano-anatase promoted SOD activity, however, are still not clearly understood. In the present work, nano-anatase in various concentrations was added to SOD from rat erythrocytes in vitro to gain insight into the mechanism of molecular interactions between nano-anatase and SOD by various spectral methods, suggesting that the reaction between SOD and nano-anatase was two-order. which meant that the SOD activity was greatly increased by low concentration of nano-anatase and inhibited by high concentration of nano-anatase. The spectroscopic assays suggested that the nano-anatase was determined to directly bind to SOD; the binding site of nano-anatase to SOD was 0.256 and the binding constants were 6.54 x 10(5) and 3.6 x 10(5) Lmol(-1); Ti was bound with three oxygen or nitrogen atoms and a Sulfur atoms of amino acid residues at the Ti-O(N) and Ti-S bond lengths of 1.86 and 2.37 angstrom, respectively, the binding nano-anatase entirely altered the secondary structure of SOD. It implied that the nano-anatase coordination created a new metal ion-active site form in SOD, thus leading to an enhancement in SOD activity. (C) 2009 Elsevier B.V. All rights reserved.
Ma, LL,Ze, YG,Liu, J,et al. Direct evidence for interaction between nano-anatase and superoxide dismutase from rat erythrocytes[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,2009,73(2):330-335.
Ma, LL.,Ze, YG.,Liu, J.,Liu, HT.,Liu, C.,...&Hong, FS.(2009).Direct evidence for interaction between nano-anatase and superoxide dismutase from rat erythrocytes.SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY,73(2),330-335.
Ma, LL,et al."Direct evidence for interaction between nano-anatase and superoxide dismutase from rat erythrocytes".SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 73.2(2009):330-335.