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Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue
Andrews, JC; Almeida, E; van der Meulen, MCH; Alwood, JS; Lee, C; Liu, YJ; Chen, J; Meirer, F; Feser, M; Gelb, J; Rudati, J; Tkachuk, A; Yun, WB; Pianetta, P; Liu YJ(刘宜晋)
2010
发表期刊MICROSCOPY AND MICROANALYSIS
卷号16期号:3页码:327-336
通讯作者[Andrews, Joy C. ; Pianetta, Piero] SLAC Natl Accelerator Lab, Stanford Synchrotron Radiat Lightsource, Menlo Pk, CA 94025 USA ; [Almeida, Eduardo] NASA, Ames Res Ctr, Moffett Field, CA 94035 USA ; [van der Meulen, Marjolein C. H.] Cornell Univ, Sibley Sch Mech & Aerosp Engn, Ithaca, NY 14853 USA ; [Alwood, Joshua S.] Stanford Univ, Dept Aeronaut & Astronaut, Stanford, CA 94305 USA ; [Lee, Chialing] San Jose State Univ, Dept Biol Sci, San Jose, CA 95192 USA ; [Liu, Yijin] Inst High Energy Phys, Beijing 100039, Peoples R China ; [Chen, Jie] Univ Sci & Technol China, Natl Synchrotron Radiat Lab, Hefei 230026, Peoples R China ; [Meirer, Florian] Vienna Univ Technol, Inst Atom & Subatom Phys, Vienna, Austria ; [Feser, Michael ; Gelb, Jeff ; Rudati, Juana ; Tkachuk, Andrei ; Yun, Wenbing] Xradia Inc, Concord, CA 94520 USA
摘要A novel hard transmission X-ray microscope (TXM) at the Stanford Synchrotron Radiation Light-source operating from 5 to 15 keV X-ray energy with 14 to 30 mu m(2) field of view has been used for high-resolution (30-40 nm) imaging and density quantification of mineralized tissue. TXM is uniquely suited for imaging of internal cellular structures and networks in mammalian mineralized tissues using relatively thick (50 mu m), untreated samples that preserve tissue micro-and nanostructure. To test this method we performed Zernike phase contrast and absorption contrast imaging of mouse cancellous bone prepared under different conditions of in vivo loading, fixation, and contrast agents. In addition, the three-dimensional structure was examined using tomography. Individual osteocytic lacunae were observed embedded within trabeculae in cancellous bone. Extensive canalicular networks were evident and included processes with diameters near the 30-40 nm instrument resolution that have not been reported previously. Trabecular density was quantified relative to rod-like crystalline apatite, and rod-like trabecular struts were found to have 51-54% of pure crystal density and plate-like areas had 44-53% of crystal density. The nanometer resolution of TXM enables future studies for visualization and quantification of ultrastructural changes in bone tissue resulting from osteoporosis, dental disease, and other pathologies.
文章类型Article
关键词X-ray microscopy imaging bone ultrastructure bone mineral density trabeculae lacuna osteocyte
学科领域Materials Science; Microscopy
DOI10.1017/S1431927610000231
研究领域[WOS]Materials Science ; Microscopy
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语种英语
WOS类目Materials Science, Multidisciplinary ; Microscopy
WOS记录号WOS:000277902600011
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被引频次:56[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/236996
专题多学科研究中心
作者单位中国科学院高能物理研究所
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Andrews, JC,Almeida, E,van der Meulen, MCH,et al. Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue[J]. MICROSCOPY AND MICROANALYSIS,2010,16(3):327-336.
APA Andrews, JC.,Almeida, E.,van der Meulen, MCH.,Alwood, JS.,Lee, C.,...&刘宜晋.(2010).Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue.MICROSCOPY AND MICROANALYSIS,16(3),327-336.
MLA Andrews, JC,et al."Nanoscale X-Ray Microscopic Imaging of Mammalian Mineralized Tissue".MICROSCOPY AND MICROANALYSIS 16.3(2010):327-336.
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