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Indirect determination of protein content by inductively coupled plasma-mass spectrometry
Wang M(王萌); Feng WY(丰伟悦); Li B(李柏); Wang B(汪冰); Zhu MT(朱墨桃); Wang Y(王云); Zhao YL(赵宇亮); Chai ZF(柴之芳); Wang, M; Feng, WY; Lu, WW; Li, B; Wang, B; Zhu, MT; Wang, Y; Zhao, YL; Chai, ZF
2008
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号36期号:3页码:321-324
通讯作者[Wang Meng ; Feng Wei-Yue ; Li Bai ; Wang Bing ; Zhu Mo-Tao ; Wang Yun ; Zhao Yu-Liang ; Chai Zhi-Fang] Chinese Acad Sci, Inst High Energy Phys, Lab Bioenvironm Effects Nanomat, Beijing 100049, Peoples R China ; [Wang Meng ; Feng Wei-Yue ; Li Bai ; Wang Bing ; Zhu Mo-Tao ; Wang Yun ; Zhao Yu-Liang ; Chai Zhi-Fang] Chinese Acad Sci, Inst High Energy Phys, Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China ; [Wang Meng ; Wang Bing ; Zhu Mo-Tao ; Wang Yun ; Zhao Yu-Liang] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China ; [Lu Wen-Wei] Shanghai Jiao Tong Univ, Shanghai 200030, Peoples R China
摘要Three standard proteins including bovine serum albumin (BSA), superoxide dismutase (SOD) and metallothionein (MT) were separated by size exclusion chromatography and on-line determined via sulfur by inductively coupled plasma-mass spectrometry. Molar ratios of the proteins were calculated by the analytical results and the known sulfur content in proteins. The mobile phase was 0.1 mol/L Tris-HAc. In order to avoid the polyatomic interferences on the sulfur determination, oxygen was added into the collision cell and reacted with sulfur to form sulfur-oxide ion. Therefore, the intensity of (SO+)-S-32-O-16 represented sulfur content during the measurement. These results were in good agreement with the gravimetric results. The RSDs for the proteins are less than 3% and the detection limits for BSA, SOD and MT-II are 14, 52 and 27 pmol, respectively.
文章类型Article
关键词sulfur inductively coupled plasma-mass spectrometry collision cell size exclusion chromatography protein quantification
学科领域Chemistry
研究领域[WOS]Chemistry
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语种英语
WOS类目Chemistry, Analytical
WOS记录号WOS:000254846900010
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被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/236965
专题院士
实验物理中心
加速器中心
多学科研究中心
作者单位中国科学院高能物理研究所
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Wang M,Feng WY,Li B,et al. Indirect determination of protein content by inductively coupled plasma-mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(3):321-324.
APA 王萌.,丰伟悦.,李柏.,汪冰.,朱墨桃.,...&Chai, ZF.(2008).Indirect determination of protein content by inductively coupled plasma-mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(3),321-324.
MLA 王萌,et al."Indirect determination of protein content by inductively coupled plasma-mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.3(2008):321-324.
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