The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin
Wang; Xingyong; Li YR(李玉茹); Li T(李婷); 刘蕾(多); Wu HC(吴海臣); Li; Yuru; Li; Ting; Liu; Lei; Wu; Hai-Chen
刊名SCIENCE CHINA-CHEMISTRY
2015
卷号59期号:1页码:135-141
关键词Biomacromolecules Current amplitude Low concentrations Secondary structures signature event Single channel recording Small organic molecules translocation
DOI10.1007/s11426-015-5455-1
通讯作者刘蕾(多) ; 吴海臣
文章类型Article
英文摘要Nanopore has been developed to be a powerful, single-molecule analytical tool for sensing ions, small organic molecules and biomacromolecules such as proteins and DNAs. Generally, the identity of the analyte can be revealed by current amplitude changes and mean dwell time of the analyte binding events. In some cases, generation of highly characteristic current events affords an alternative way of analyte determination with high confidence level. However, we found that secondary structures in DNA/RNA hybrids might severely hinder the generation of signature events during their translocation through α-hemolysin nanopore. In this report, we propose a strategy to add a certain concentration of urea in the buffer solution for single channel recordings and validate that low concentration of urea can effectively denature the secondary structures in DNA hybrids and recover the generation of signature events. This finding might be useful in other secondary structure-related nanopore sensing activities. © 2015 Science China Press and Springer-Verlag Berlin Heidelberg
类目[WOS]461.2 Biological Materials and Tissue Engineering ; 461.9 Biology ; 761 Nanotechnology ; 804.1 Organic Compounds ; 931.3 Atomic and Molecular Physics ; 933 Solid State Physics
收录类别EI ; CA
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被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228916
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_核技术应用研究中心
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Wang,Xingyong,Li YR,et al. The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin[J]. SCIENCE CHINA-CHEMISTRY,2015,59(1):135-141.
APA Wang.,Xingyong.,李玉茹.,李婷.,刘蕾.,...&Hai-Chen.(2015).The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin.SCIENCE CHINA-CHEMISTRY,59(1),135-141.
MLA Wang,et al."The effect of secondary structures on the generation of characteristic events during the translocation of DNA hybrid through α-hemolysin".SCIENCE CHINA-CHEMISTRY 59.1(2015):135-141.
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