Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.

doi:10.1021/acsnano.5b04261

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	Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.
	Gao J(高靓); Liu MQ(刘梅青); Ma GF(马桂服); Wang YL(王亚玲); Zhao LN(赵丽娜); Yuan Q(袁卿); Gao FP(高福平); Liu R(刘茹); Zhai J(翟皎); Chai ZF(柴之芳); Zhao YL(赵宇亮); Gao XY(高学云); Gao; Liang; Liu; Meiqing; Ma; Guifu; Wang; Yaling; Zhao; Lina; Yuan; Qing; Gao; Fuping; Liu; Ru; Zhai; Jiao; Chai; Zhifang; Zhao; Yuliang; Gao; Xueyun.
	2015
发表期刊	ACS NANO (IF:13.942[JCR-2016],14.194[5-Year])
ISSN	1936-0851
EISSN	1936-086X
卷号	9 期号:11 页码:10979-10990
通讯作者	高学云
文章类型	Article
摘要	Precisely quantifying the membrane protein expression level on cell surfaces is of vital importance for early cancer diagnosis and efficient treatment. We demonstrate that gold nanoparticle bioconjugated by a rationally designed peptide as nanoprobe possesses selective labeling and accurate quantification capacity of integrin GPIlb/IIIa on the human erythroleukemia cell line. Through selective recognition and marking of integrin, two-photon photoluminescence of the nanoprobe is exploited for direct observation of protein spatial distribution on cell membrane. More importantly, utilizing intrinsic enzyme-like catalysis property of the nanoprobe, the expression level of integrin on human erythroleukemia cells can be quantitatively counted in an amplified and reliable colorimetric assay without cell lysis and protein extraction process. In addition, the analysis of the correlation between the gold nanoparticle and the membrane protein via relevant inductively coupled plasma mass spectrometry measurement verifies the reliability of the new analytical method. It is anticipated that this facile and efficient strategy holds a great promise for a rapid, precise, and reliable quantification of interested functional membrane proteins on the cell surface.
关键词	nanozyme enzyme-linked immunosorbent assay gold nanoparticles membrane protein quantification two-photon imaging
学科领域	Chemistry; Science & Technology - Other Topics; Materials Science
DOI	10.1021/acsnano.5b04261
收录类别	SCI ; EI ; CA
语种	英语
WOS研究方向	Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS类目	Chemistry ; Science & Technology - Other Topics ; Materials Science
WOS记录号	WOS:000365464800046
引用统计	正在获取...
文献类型	期刊论文
条目标识符	https://ir.ihep.ac.cn/handle/311005/228877
专题	院士多学科研究中心
推荐引用方式 GB/T 7714	Gao J,Liu MQ,Ma GF,et al. Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.[J]. ACS NANO,2015,9(11):10979-10990.
APA	高靓.,刘梅青.,马桂服.,王亚玲.,赵丽娜.,...&Xueyun..(2015).Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane..ACS NANO,9(11),10979-10990.
MLA	高靓,et al."Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.".ACS NANO 9.11(2015):10979-10990.