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Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.
Gao J(高靓); Liu MQ(刘梅青); Ma GF(马桂服); Wang YL(王亚玲); Zhao LN(赵丽娜); Yuan Q(袁卿); Gao FP(高福平); Liu R(刘茹); Zhai J(翟皎); Chai ZF(柴之芳); Zhao YL(赵宇亮); Gao XY(高学云); Gao; Liang; Liu; Meiqing; Ma; Guifu; Wang; Yaling; Zhao; Lina; Yuan; Qing; Gao; Fuping; Liu; Ru; Zhai; Jiao; Chai; Zhifang; Zhao; Yuliang; Gao; Xueyun.
2015
Source PublicationACS NANO
ISSN1936-0851
EISSN1936-086X
Volume9Issue:11Pages:10979-10990
Corresponding Author高学云
SubtypeArticle
AbstractPrecisely quantifying the membrane protein expression level on cell surfaces is of vital importance for early cancer diagnosis and efficient treatment. We demonstrate that gold nanoparticle bioconjugated by a rationally designed peptide as nanoprobe possesses selective labeling and accurate quantification capacity of integrin GPIlb/IIIa on the human erythroleukemia cell line. Through selective recognition and marking of integrin, two-photon photoluminescence of the nanoprobe is exploited for direct observation of protein spatial distribution on cell membrane. More importantly, utilizing intrinsic enzyme-like catalysis property of the nanoprobe, the expression level of integrin on human erythroleukemia cells can be quantitatively counted in an amplified and reliable colorimetric assay without cell lysis and protein extraction process. In addition, the analysis of the correlation between the gold nanoparticle and the membrane protein via relevant inductively coupled plasma mass spectrometry measurement verifies the reliability of the new analytical method. It is anticipated that this facile and efficient strategy holds a great promise for a rapid, precise, and reliable quantification of interested functional membrane proteins on the cell surface.
Keywordnanozyme enzyme-linked immunosorbent assay gold nanoparticles membrane protein quantification two-photon imaging
Subject AreaChemistry; Science & Technology - Other Topics; Materials Science
DOI10.1021/acsnano.5b04261
Indexed BySCI ; EI ; CA
Language英语
WOS Research AreaChemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS SubjectChemistry ; Science & Technology - Other Topics ; Materials Science
WOS IDWOS:000365464800046
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/228877
Collection院士
多学科研究中心
Recommended Citation
GB/T 7714
Gao J,Liu MQ,Ma GF,et al. Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.[J]. ACS NANO,2015,9(11):10979-10990.
APA 高靓.,刘梅青.,马桂服.,王亚玲.,赵丽娜.,...&Xueyun..(2015).Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane..ACS NANO,9(11),10979-10990.
MLA 高靓,et al."Peptide-Conjugated Gold Nanoprobe: Intrinsic Nanozyme-Linked Immunosorbent Assay of Integrin Expression Level on Cell Membrane.".ACS NANO 9.11(2015):10979-10990.
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