Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores
Ceng T(曾涛); 刘蕾(多); Li T(李婷); Li YR(李玉茹); Gao J(高娟); Wu HC(吴海臣); Zeng, T; Liu, L; Li, T; Li, YR; Gao, J; Zhao, YL; Wu, HC
刊名CHEMICAL SCIENCE
2015
卷号6期号:10页码:5628-5634
学科分类Chemistry
DOI10.1039/c5sc01436k
通讯作者吴海臣
文章类型Article
英文摘要Cytosine methylation and hydroxymethylation are both important epigenetic modifications of DNA in mammalian cells. Therefore, profiling DNA (hydroxy) methylation across the genome is vital for understanding their roles in gene regulation. Here, we report a nanopore-based approach for quick and reliable detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA at the single-molecule level. The single-stranded DNA containing 5-methylcytosine or 5-hydroxymethylcytosine was first selectively modified on the epigenetic base to attach a host-guest complex. Threading of the modified DNA molecules through a-hemolysin nanopores causes unbinding of the host-guest complex and generates highly characteristic current signatures. Statistical analysis of the signature events affords quantitative information about 5-methylcytosine and 5-hydroxymethylcytosine in DNA. Our results suggest that other DNA modifications could also be detected with the developed method. Furthermore, we anticipate our nanopore sensing strategy to be generally useful in biochemical analysis and to find applications in the early diagnosis of diseases.
类目[WOS]Chemistry, Multidisciplinary
收录类别SCI ; EI ; CA
WOS记录号WOS:000361212000033
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228750
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_核技术应用研究中心
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Ceng T,Liu L,Li T,et al. Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores[J]. CHEMICAL SCIENCE,2015,6(10):5628-5634.
APA 曾涛.,刘蕾.,李婷.,李玉茹.,高娟.,...&Wu, HC.(2015).Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores.CHEMICAL SCIENCE,6(10),5628-5634.
MLA 曾涛,et al."Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores".CHEMICAL SCIENCE 6.10(2015):5628-5634.
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