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Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores
Ceng T(曾涛); 刘蕾(多); Li T(李婷); Li YR(李玉茹); Gao J(高娟); Wu HC(吴海臣); Zeng, T; Liu, L; Li, T; Li, YR; Gao, J; Zhao, YL; Wu, HC
2015
发表期刊CHEMICAL SCIENCE
卷号6期号:10页码:5628-5634
通讯作者吴海臣
文章类型Article
摘要Cytosine methylation and hydroxymethylation are both important epigenetic modifications of DNA in mammalian cells. Therefore, profiling DNA (hydroxy) methylation across the genome is vital for understanding their roles in gene regulation. Here, we report a nanopore-based approach for quick and reliable detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA at the single-molecule level. The single-stranded DNA containing 5-methylcytosine or 5-hydroxymethylcytosine was first selectively modified on the epigenetic base to attach a host-guest complex. Threading of the modified DNA molecules through a-hemolysin nanopores causes unbinding of the host-guest complex and generates highly characteristic current signatures. Statistical analysis of the signature events affords quantitative information about 5-methylcytosine and 5-hydroxymethylcytosine in DNA. Our results suggest that other DNA modifications could also be detected with the developed method. Furthermore, we anticipate our nanopore sensing strategy to be generally useful in biochemical analysis and to find applications in the early diagnosis of diseases.
学科领域Chemistry
DOI10.1039/c5sc01436k
收录类别SCI ; EI ; CA
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000361212000033
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228750
专题多学科研究中心
核技术应用研究中心
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GB/T 7714
Ceng T,Liu L,Li T,et al. Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores[J]. CHEMICAL SCIENCE,2015,6(10):5628-5634.
APA 曾涛.,刘蕾.,李婷.,李玉茹.,高娟.,...&Wu, HC.(2015).Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores.CHEMICAL SCIENCE,6(10),5628-5634.
MLA 曾涛,et al."Detection of 5-methylcytosine and 5-hydroxymethylcytosine in DNA via host-guest interactions inside alpha-hemolysin nanopores".CHEMICAL SCIENCE 6.10(2015):5628-5634.
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