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3D imaging of a rice pollen grain using transmission X-ray microscopy
Wang, SX; Wang, DJ; Wu, Q; Gao, K; Wang, ZL; Wu, ZY; Wu ZY(吴自玉)
Corresponding Author吴自玉
AbstractFor the first time, the three-dimensional (3D) ultrastructure of an intact rice pollen cell has been obtained using a full-field transmission hard X-ray microscope operated in Zernike phase contrast mode. After reconstruction and segmentation from a series of projection images, complete 3D structural information of a 35 mu m rice pollen grain is presented at a resolution of similar to 100nm. The reconstruction allows a clear differentiation of various subcellular structures within the rice pollen grain, including aperture, lipid body, mitochondrion, nucleus and vacuole. Furthermore, quantitative information was obtained about the distribution of cytoplasmic organelles and the volume percentage of each kind of organelle. These results demonstrate that transmission X-ray microscopy can be quite powerful for non-destructive investigation of 3D structures of whole eukaryotic cells.
Keywordtransmission X-ray microscopy nano-tomography rice pollen grain cellular microstructure
Subject AreaInstruments & Instrumentation; Optics; Physics
Indexed BySCI ; EI
WOS Research AreaInstruments & Instrumentation ; Optics ; Physics, Applied
WOS SubjectInstruments & Instrumentation ; Optics ; Physics
WOS IDWOS:000357407900028
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Document Type期刊论文
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GB/T 7714
Wang, SX,Wang, DJ,Wu, Q,et al. 3D imaging of a rice pollen grain using transmission X-ray microscopy[J]. JOURNAL OF SYNCHROTRON RADIATION,2015,22:1091-1095.
APA Wang, SX.,Wang, DJ.,Wu, Q.,Gao, K.,Wang, ZL.,...&吴自玉.(2015).3D imaging of a rice pollen grain using transmission X-ray microscopy.JOURNAL OF SYNCHROTRON RADIATION,22,1091-1095.
MLA Wang, SX,et al."3D imaging of a rice pollen grain using transmission X-ray microscopy".JOURNAL OF SYNCHROTRON RADIATION 22(2015):1091-1095.
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