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3D imaging of a rice pollen grain using transmission X-ray microscopy
Wang, SX; Wang, DJ; Wu, Q; Gao, K; Wang, ZL; Wu, ZY; Wu ZY(吴自玉)
2015
发表期刊JOURNAL OF SYNCHROTRON RADIATION
卷号22页码:1091-1095
通讯作者吴自玉
摘要For the first time, the three-dimensional (3D) ultrastructure of an intact rice pollen cell has been obtained using a full-field transmission hard X-ray microscope operated in Zernike phase contrast mode. After reconstruction and segmentation from a series of projection images, complete 3D structural information of a 35 mu m rice pollen grain is presented at a resolution of similar to 100nm. The reconstruction allows a clear differentiation of various subcellular structures within the rice pollen grain, including aperture, lipid body, mitochondrion, nucleus and vacuole. Furthermore, quantitative information was obtained about the distribution of cytoplasmic organelles and the volume percentage of each kind of organelle. These results demonstrate that transmission X-ray microscopy can be quite powerful for non-destructive investigation of 3D structures of whole eukaryotic cells.
文章类型Article
关键词transmission X-ray microscopy nano-tomography rice pollen grain cellular microstructure
学科领域Instruments & Instrumentation; Optics; Physics
DOI10.1107/S1600577515009716
收录类别SCI ; EI
WOS类目Instruments & Instrumentation ; Optics ; Physics, Applied
WOS记录号WOS:000357407900028
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228621
专题多学科研究中心
推荐引用方式
GB/T 7714
Wang, SX,Wang, DJ,Wu, Q,et al. 3D imaging of a rice pollen grain using transmission X-ray microscopy[J]. JOURNAL OF SYNCHROTRON RADIATION,2015,22:1091-1095.
APA Wang, SX.,Wang, DJ.,Wu, Q.,Gao, K.,Wang, ZL.,...&吴自玉.(2015).3D imaging of a rice pollen grain using transmission X-ray microscopy.JOURNAL OF SYNCHROTRON RADIATION,22,1091-1095.
MLA Wang, SX,et al."3D imaging of a rice pollen grain using transmission X-ray microscopy".JOURNAL OF SYNCHROTRON RADIATION 22(2015):1091-1095.
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