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Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts
Zhao JT(赵甲亭); Gao YX(高愈希); Peng XM(彭晓敏); Li YY(李云云); Xu XH(徐小晗); Li B(李柏); Dong JC(董俊才); Li YF(李玉锋); Zhao, JT; Pu, YX; Gao, YX; Peng, XM; Li, YY; Xu, XH; Li, B; Zhu, NL; Dong, JC; Wu, G; Li, YF
2015
发表期刊JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷号30期号:6页码:1408-1413
通讯作者李玉锋
摘要A comprehensive approach that can identify and quantify selenium (Se) in seleno-proteins in Se-enriched yeast was developed. The Se-containing compounds in Se-enriched yeast were first extracted and then the fraction of Se-containing proteins in the supernatant was analyzed by 2-dimensional electrophoresis (2-DE) and synchrotron radiation X-ray fluorescence (SR-XRF). The detection limit (DL) of SR-XRF analysis for Se quantification in Se-containing proteins after 2-DE separation was calculated to be 0.20 mu g g(-1), which is suitable for Se quantification in the Se-containing spots present on the 2-D gel. After being scanned by SR-XRF, only spots with a mean Se content exceeding twice the DL of SR-XRF were considered to be seleno-proteins. In this way, a total of 157 Se-containing spots in the gel were visually distinguished. Se contents in all the Se-containing proteins of different molecular weights were quantified. The total Se content on the 2-D gel was calculated to be 126.56 mu g g(-1), which covered most of the seleno-proteins on the 2-D gel.
文章类型Article
学科领域Chemistry; Spectroscopy
DOI10.1039/c5ja00043b
收录类别SCI ; EI ; CA
WOS类目Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000355559500021
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228479
专题多学科研究中心
实验物理中心
推荐引用方式
GB/T 7714
Zhao JT,Gao YX,Peng XM,et al. Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2015,30(6):1408-1413.
APA 赵甲亭.,高愈希.,彭晓敏.,李云云.,徐小晗.,...&Li, YF.(2015).Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,30(6),1408-1413.
MLA 赵甲亭,et al."Identification and quantification of seleno-proteins by 2-DE-SR-XRF in selenium-enriched yeasts".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 30.6(2015):1408-1413.
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