Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions

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	Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions
其他题名	激光光散射测量荧光/磷光体系溶液结构的原理和方法
	Jia, D; Yang, JF; Cheng H(程贺); Cheng, H; Han, CC
	2015
发表期刊	ACTA POLYMERICA SINICA
期号	5 页码:564-570
通讯作者	程贺
文章类型	Article
摘要	As a typical elastic scattering technique,laser light scattering results cannot precisely describe the solution structure when the samples absorb incident light. Therefore, commercial laser light scattering instrument has been modified and upgraded to utilize four independent lasers, e. g. 785 nm,632.8 nm,532 nm and 457 nm, respectively as the light sources. By switching the wavelengths of incident lasers to avoid the sample absorption, the instrument can be widely used in fluorescent/phosphorescence solution systems. Spherical polystyrene nanoparticles with and without fluorescent dyes are used to calibrate the modified instrument. It is found that the height of the normalized intensity-intensity time correlation function decreases with the increase of the scattering angle when the sample absorbs the incident laser light. Such a correlation function fails to describe the sample solution structure, because the corresponding hydrodynamic radius distribution shows two modes and the average characteristic line width versus the square of scattering vector q(2) does not fit a good linear relationship any more. Therefore, another laser which is not absorbed by the sample is used to do the calibration again. The corresponding intensity-intensity time correlation functions and the fitting results can precisely describe the solution structure. Fluorescence correlation spectroscopy (FCS) is also conducted to further prove the results obtained from the modified laser light scattering instrument are precise and accurate.
关键词	Laser light scattering Fluorescent/phosphorescence Fluorescence correlation spectroscopy (FCS) Quasi-elastic scattering
学科领域	Polymer Science
收录类别	SCI
WOS类目	Polymer Science
WOS记录号	WOS:000355574800010
引用统计	被引频次：5[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.ihep.ac.cn/handle/311005/228473
专题	东莞分部实验物理中心
推荐引用方式 GB/T 7714	Jia, D,Yang, JF,Cheng H,et al. Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions[J]. ACTA POLYMERICA SINICA,2015(5):564-570.
APA	Jia, D,Yang, JF,程贺,Cheng, H,&Han, CC.(2015).Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions.ACTA POLYMERICA SINICA(5),564-570.
MLA	Jia, D,et al."Basic Principle of SLS/DLS Measurements in Fluorescent/Phosphorescence Solutions".ACTA POLYMERICA SINICA .5(2015):564-570.

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