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A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin
Li T(李婷); 刘蕾(多); Li YR(李玉茹); Xie JN(谢佳妮); Wu HC(吴海臣); Li, T; Liu, L; Li, YR; Xie, JN; Wu, HC
2015
发表期刊ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷号54期号:26页码:7678-7681
通讯作者吴海臣
文章类型Article
摘要Nanopore emerged as a powerful single-molecule technique over the past two decades, and has shown applications in the stochastic sensing and biophysical studies of individual molecules. Here, we report a versatile strategy for nanopore sensing by employing the combination of aptamers and host-guest interactions. An aptamer is first hybridized with a DNA probe which is modified with a ferrocene< subset of>cucurbit[7]uril complex. The presence of analytes causes the aptamer-probe duplex to unwind and release the DNA probe which can quantitatively produce signature current events when translocated through an -hemolysin nanopore. The integrated use of magnetic beads can further lower the detection limit by approximately two to three orders of magnitude. Because aptamers have shown robust binding affinities with a wide variety of target molecules, our proposed strategy should be universally applicable for sensing different types of analytes with nanopore sensors.
关键词aptamers host-guest systems magnetic beads nanopore sensors quantitative determination
学科领域Chemistry
DOI10.1002/anie.201502047
收录类别SCI ; EI ; CA
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000356390900018
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228413
专题多学科研究中心
核技术应用研究中心
推荐引用方式
GB/T 7714
Li T,Liu L,Li YR,et al. A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2015,54(26):7678-7681.
APA 李婷.,刘蕾.,李玉茹.,谢佳妮.,吴海臣.,...&Wu, HC.(2015).A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,54(26),7678-7681.
MLA 李婷,et al."A Universal Strategy for Aptamer-Based Nanopore Sensing through Host-Guest Interactions inside -Hemolysin".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 54.26(2015):7678-7681.
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