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Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell
Zhai J(翟蛟); Wang YL(王亚玲); Xu C(徐超); Zheng LN(郑令娜); Wang M(王萌); Feng WY(丰伟悦); Gao J(高靓); Zhao LN(赵丽娜); Liu R(刘茹); Gao FP(高福平); Zhao YL(赵宇亮); Chai ZF(柴之芳); Gao XY(高学云); Zhai, J; Wang, YL; Xu, C; Zheng, LN; Wang, M; Feng, WY; Gao, L; Zhao, LN; Liu, R; Gao, FP; Zhao, YL; Chai, ZF; Gao, XY
2015
Source PublicationANALYTICAL CHEMISTRY
ISSN0003-2700
EISSN1520-6882
Volume87Issue:5Pages:2546-2549
Corresponding Author王亚玲 ; 高学云
SubtypeArticle
AbstractWe report for the first time seeing and counting integrin a(IIb)beta(3) on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au-24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin a(IIb)beta(3) on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au(24)Peptide(8)), the number of integrin a(IIb)beta(3) can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin a(IIb)beta(3) on a single cell varies from 5.75 x 10(-17) to 9.11 x 10(-17) mol, because of the heteroexpression levels of a(IIb)beta(3) on individual cells.
Subject AreaChemistry
DOI10.1021/ac504639u
Indexed BySCI ; EI ; CA
Language英语
WOS Research AreaChemistry, Analytical
WOS SubjectChemistry
WOS IDWOS:000350611700006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/228242
Collection院士
多学科研究中心
Recommended Citation
GB/T 7714
Zhai J,Wang YL,Xu C,et al. Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell[J]. ANALYTICAL CHEMISTRY,2015,87(5):2546-2549.
APA 翟蛟.,王亚玲.,徐超.,郑令娜.,王萌.,...&Gao, XY.(2015).Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell.ANALYTICAL CHEMISTRY,87(5),2546-2549.
MLA 翟蛟,et al."Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell".ANALYTICAL CHEMISTRY 87.5(2015):2546-2549.
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