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Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell
Zhai J(翟蛟); Wang YL(王亚玲); Xu C(徐超); Zheng LN(郑令娜); Wang M(王萌); Feng WY(丰伟悦); Gao J(高靓); Zhao LN(赵丽娜); Liu R(刘茹); Gao FP(高福平); Zhao YL(赵宇亮); Chai ZF(柴之芳); Gao XY(高学云); Zhai, J; Wang, YL; Xu, C; Zheng, LN; Wang, M; Feng, WY; Gao, L; Zhao, LN; Liu, R; Gao, FP; Zhao, YL; Chai, ZF; Gao, XY
2015
发表期刊ANALYTICAL CHEMISTRY
卷号87期号:5页码:2546-2549
通讯作者王亚玲 ; 高学云
文章类型Article
摘要We report for the first time seeing and counting integrin a(IIb)beta(3) on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au-24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin a(IIb)beta(3) on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au(24)Peptide(8)), the number of integrin a(IIb)beta(3) can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin a(IIb)beta(3) on a single cell varies from 5.75 x 10(-17) to 9.11 x 10(-17) mol, because of the heteroexpression levels of a(IIb)beta(3) on individual cells.
学科领域Chemistry
DOI10.1021/ac504639u
收录类别SCI ; EI ; CA
WOS类目Chemistry, Analytical
WOS记录号WOS:000350611700006
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被引频次:20[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228242
专题院士
加速器中心
多学科研究中心
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Zhai J,Wang YL,Xu C,et al. Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell[J]. ANALYTICAL CHEMISTRY,2015,87(5):2546-2549.
APA 翟蛟.,王亚玲.,徐超.,郑令娜.,王萌.,...&Gao, XY.(2015).Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell.ANALYTICAL CHEMISTRY,87(5),2546-2549.
MLA 翟蛟,et al."Facile Approach To Observe and Quantify the alpha(IIb)beta(3) Integrin on a Single-Cell".ANALYTICAL CHEMISTRY 87.5(2015):2546-2549.
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