IHEP OpenIR  > 院士
Quantifying the distribution of ceria nanoparticles in cucumber roots: the influence of labeling
He X(何潇); Ma YH(马宇辉); Li YY(李媛媛); Ding YY(丁雅韵); Chai ZF(柴之芳); Zhao YL(赵宇亮); Zhang ZY(张智勇); Gui, X; He, X; Ma, YH; Zhang, P; Li, YY; Ding, YY; Yang, K; Li, HF; Rui, YK; Chai, ZF; Zhao, YL; Zhang, ZY
2015
发表期刊RSC ADVANCES
卷号5期号:6页码:4554-4560
通讯作者张智勇 ; 何潇
摘要Monitoring the fate of nanoparticles (NPs) in the environment and organisms is always the first step towards better understanding the mechanisms for their toxicity. Fluorescent labeling is one of the most common methods to detect the NPs in samples, however, the attachment of a fluorescent tag to NPs might cause unwanted changes in the distribution and bio-effects of NPs. In the present work, the distribution of ceria nanoparticles (nano-ceria) in cucumber roots after fluorescent labeling, as well as the influence of labeling, was studied. Nano-ceria sized 6.6 nm was labeled by surface coating with diiodofluorescein (DIF), so that the distribution of NPs and the fluorophore could be determined simultaneously by micro-synchrotron radiation X-ray fluorescence (mu-SRXRF) analysis. The two-dimensional mappings of Ce and I in the roots treated with DIF, nano-ceria, and DIF-coated nano-ceria were compared with each other. DIF-coating, though only 25% of the particulate surface was coated, might alter the surface properties of nano-ceria, thereby changing its distribution in cucumber. The co-existence of nano-ceria and DIF greatly enhanced the contact between seeds and DIF, increased the local concentration of DIF on the root surface, and exacerbated the phytotoxicity of DIF. To the best of our knowledge, this is the first study focusing on how the labeling protocol affects the distribution and bio-effects of both the labeling tag and the NPs. Therefore, a guideline on the applicability of the labeling protocol should be developed to ensure the nanotoxicological data obtained using labeling techniques are precise and reliable.
文章类型Article
学科领域Chemistry
DOI10.1039/c4ra13915a
收录类别SCI ; EI
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000346570300080
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228207
专题院士
多学科研究中心
推荐引用方式
GB/T 7714
He X,Ma YH,Li YY,et al. Quantifying the distribution of ceria nanoparticles in cucumber roots: the influence of labeling[J]. RSC ADVANCES,2015,5(6):4554-4560.
APA 何潇.,马宇辉.,李媛媛.,丁雅韵.,柴之芳.,...&Zhang, ZY.(2015).Quantifying the distribution of ceria nanoparticles in cucumber roots: the influence of labeling.RSC ADVANCES,5(6),4554-4560.
MLA 何潇,et al."Quantifying the distribution of ceria nanoparticles in cucumber roots: the influence of labeling".RSC ADVANCES 5.6(2015):4554-4560.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
139.pdf(544KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[何潇]的文章
[马宇辉]的文章
[李媛媛]的文章
百度学术
百度学术中相似的文章
[何潇]的文章
[马宇辉]的文章
[李媛媛]的文章
必应学术
必应学术中相似的文章
[何潇]的文章
[马宇辉]的文章
[李媛媛]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 139.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。