Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection
Ma L(马磊); Tang BC(汤博崇); Yang WJ(杨文江); 刘宇(多); Zhao YL(赵宇亮); Li M(李敏); Ma, L; Tang, BC; Yang, WJ; Liu, Y; Zhao, YL; Li, M
刊名ANALYTICAL METHODS
2015
卷号7期号:4页码:1622-1626
学科分类Chemistry; Food Science & Technology; Spectroscopy
DOI10.1039/c4ay02783c
通讯作者赵宇亮 ; 李敏
文章类型Article
英文摘要In the present work, we integrated a prepared bio-chip with Tc-99m labeling to improve the immunoassay for cancer biomarker protein detection. Efficient binding of a Tc-99m isotope-labeled secondary antibody to the biomarker protein pre-anchored to the bio-chip was followed by detection of g rays from Tc-99m decay via a Gamma Well Counter. We show that alpha-fetoprotein (AFP), a biomarker of liver cancer, can be detected down to 10(-18) M concentration in a buffer solution with good reproducibility. This ultra-high sensitivity is attributed to the aggregation of the Tc-99m isotope-labeled secondary antibody which greatly amplifies the g rays emitted per AFP binding event. The tendency of the isotope-labeled secondary antibody to aggregate was indicated by dynamic light scattering studies on a rhenium/secondary antibody conjugate, a stable structural analogue of the Tc-99m conjugate. The successful zeptomolar-range detection of cancer biomarkers opens new avenues for early disease diagnoses via integrating bio-chips with isotope-labeling techniques.
类目[WOS]Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
收录类别SCI ; EI ; CA
WOS记录号WOS:000349679900051
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被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228167
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
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Ma L,Tang BC,Yang WJ,et al. Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection[J]. ANALYTICAL METHODS,2015,7(4):1622-1626.
APA 马磊.,汤博崇.,杨文江.,刘宇.,赵宇亮.,...&Li, M.(2015).Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection.ANALYTICAL METHODS,7(4),1622-1626.
MLA 马磊,et al."Integration of a bio-chip technique with technetium-99m labeling provides zeptomolar sensitivity in liver cancer biomarker detection".ANALYTICAL METHODS 7.4(2015):1622-1626.
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