IHEP OpenIR
Crystal structure of the Locusta migratoria odorant binding protein
Zheng, JG; Li, JR; Han, L; Wang, Y; Wu, W; Qi, XX; Tao, Y; Zhang, L; Zhang, ZD; Chen, ZZ; Tao Y(陶冶)
2015
Source PublicationBIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
ISSN0006-291X
EISSN1090-2104
Volume456Issue:3Pages:737-742
SubtypeArticle
AbstractLocusta migratoria (Lmig) causes enormous losses to agricultural products, especially because it often infests the world with great swarms as locust plagues. Locusts find their plant hosts on which they feed through their olfactory system, in which odorant binding proteins (OBPs) play an important role. Previous study indicated that the amino acid sequences of LmigOBP showed low similarity to OBPs from other insect orders and we speculated that it might perform unique binding behavior. Here, we solved the first LmigOBP1 structure at 1.65 angstrom, which is a monomer in solution and disulfide bonds play a key role in maintaining its function. We show that LmigOBP1 possesses a unique seventh a-helix, which is located at the surface with strong interactions with the LmigOBP1 scaffold consisting of other six a-helices. Moreover, the seventh a-helix forms a wall of an "L" shaped internal hydrophobic cavity to accommodate linear ligands, which is consistent with the binding experiments. We also demonstrate that the ligand-binding pocket in LmigOBP1 is greatly different from that in the closest homologs mosquito OBPs. Taken together, this study provides a structural basis for designing small inhibitors to control locust. (C) 2014 Elsevier Inc. All rights reserved.
KeywordLocusta migratoria Odorant binding protein Odorant perception Crystal structure Ligand binding mechanism
Subject AreaBiochemistry & Molecular Biology; Biophysics
DOI10.1016/j.bbrc.2014.12.048
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Biophysics
WOS SubjectBiochemistry & Molecular Biology ; Biophysics
WOS IDWOS:000348973300006
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihep.ac.cn/handle/311005/228138
Collection中国科学院高能物理研究所
Recommended Citation
GB/T 7714
Zheng, JG,Li, JR,Han, L,et al. Crystal structure of the Locusta migratoria odorant binding protein[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2015,456(3):737-742.
APA Zheng, JG.,Li, JR.,Han, L.,Wang, Y.,Wu, W.,...&陶冶.(2015).Crystal structure of the Locusta migratoria odorant binding protein.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,456(3),737-742.
MLA Zheng, JG,et al."Crystal structure of the Locusta migratoria odorant binding protein".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 456.3(2015):737-742.
Files in This Item:
File Name/Size DocType Version Access License
69.pdf(1533KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zheng, JG]'s Articles
[Li, JR]'s Articles
[Han, L]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zheng, JG]'s Articles
[Li, JR]'s Articles
[Han, L]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zheng, JG]'s Articles
[Li, JR]'s Articles
[Han, L]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.