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An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells
Zhang, HT; Xie, YS; Wang, P; Chen, GC; Liu, RC; Lam, YW; Hu, Y; Zhu, Q; Sun, HY; Hu Y(胡毅)
2015
发表期刊TALANTA
卷号135页码:149-154
文章类型Article
摘要Hydrogen sulfide (H2S) has recently been identified as the third gaseous signaling molecule that is involved in regulating many important cellular processes. We report herein a novel fluorescent probe for detecting H2S based on iminocoumarin benzothiazole scaffold. The probe displayed high sensitivity and around 80-fold increment in fluorescence signal after reacting with H2S under physiological condition. The fluorescent intensity of the probe was linearly related to H2S concentration in the range of 0-100 mu M with a detection limit of 0.15 mu M (3 sigma/slope). The probe also showed excellent selectivity towards H2S over other biologically relevant species, including ROS, RSS and RNS. Its selectivity for H2S is 32 folds higher than other reactive sulfur species. Furthermore, the probe has been applied for imaging H2S in living cells. Cell imaging experiments demonstrated that the probe is cell-permeable and can be used to monitor the alteration of H2S concentrations in living cells. We envisage that this probe can provide useful tools to further elucidate the biological roles of H2S. (C) 2015 Elsevier B.V. All rights reserved.
关键词Fluorescent probe Hydrogen sulfide Cell imaging Iminocoumarin benzothiazole
学科领域Chemistry
DOI10.1016/j.talanta.2014.12.044
收录类别SCI ; EI
WOS类目Chemistry, Analytical
WOS记录号WOS:000349730700022
引用统计
被引频次:17[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/228072
专题多学科研究中心
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GB/T 7714
Zhang, HT,Xie, YS,Wang, P,et al. An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells[J]. TALANTA,2015,135:149-154.
APA Zhang, HT.,Xie, YS.,Wang, P.,Chen, GC.,Liu, RC.,...&胡毅.(2015).An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells.TALANTA,135,149-154.
MLA Zhang, HT,et al."An iminocoumarin benzothiazole-based fluorescent probe for imaging hydrogen sulfide in living cells".TALANTA 135(2015):149-154.
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