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A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin
Liu NQ(刘年庆); Liu PS(刘平生); Wang KJ(王孔江); Xu Q(徐清); Liu, NQ; Liu, PS; Wang, KJ; Chen, DF; Zhao, JY; Xu, Q
2000
发表期刊BIOLOGICAL TRACE ELEMENT RESEARCH
卷号76期号:3页码:#REF!
通讯作者Liu, NQ (reprint author), Acad Sinica, Inst High Energy Phys, Beijing 100080, Peoples R China.
文章类型Article
摘要This article presents an analytical method for the determination of the relative concentrations of trace elements in plasma protein by gel chromatography combined with SXRF (synchrotron radiation X-ray fluorescence). The fraction of plasma protein of male Kunming mice (body weight 24.2 +/- 0.3 g), treated with a cisplatin ip injection at a dose of 10 mg/kg, was obtained by the separation of a Sephadex G-50 column (buffered with ammonium acetate, pH 5.7). The SXRF experiments were performed at the Beijing Electron Positron Collider synchrotron radiation facility. The elements (Pt, S, Ca, Fe, Ni, Cu, Zn, Se, Br, and Sr) in the fraction of the plasma proteins (>22 kDa) were assayed using highly sensitive SXRF. The relative concentrations of elements were calculated by a normalization of Compton scattering intensity around 22 keV, after the normalization for the collection time of the X-ray spectrum and the counting of the ion chamber, and subtracting the contribution of the polycarbonate film for the supporting sample. The determination could prove that the element Pt in plasma was bound with macromolecular protein. Cu and S were present in the fraction of the protein in mice treated with cisplatin increase, and their ratios of treated/control were 1.66 +/- 0.06 and 1.78 +/- 0.33, respectively; Zn decreased to a ratio of 0.78 +/- 0.09. Our results are in agreement with others that cisplatin exposure leads to a marked loss of kidney copper and a moderate rise in kidney zinc. However, this article primarily describes one of the analytical methods used; it does not emphasize the results of the effect of cisplatin on trace elements in plasma protein.
关键词trace elements mouse plasma protein SXRF gel chromatography cisplatin
学科领域Biochemistry & Molecular Biology; Endocrinology & Metabolism
DOI10.1385/BTER:76:3:279
收录类别SCI
WOS类目Biochemistry & Molecular Biology ; Endocrinology & Metabolism
WOS记录号WOS:000089471700008
引用统计
被引频次:13[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/227369
专题多学科研究中心
推荐引用方式
GB/T 7714
Liu NQ,Liu PS,Wang KJ,et al. A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2000,76(3):#REF!.
APA 刘年庆.,刘平生.,王孔江.,徐清.,Liu, NQ.,...&Xu, Q.(2000).A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin.BIOLOGICAL TRACE ELEMENT RESEARCH,76(3),#REF!.
MLA 刘年庆,et al."A SXRF method for determining the relative concentration of trace elements in plasma protein affected by cisplatin".BIOLOGICAL TRACE ELEMENT RESEARCH 76.3(2000):#REF!.
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