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Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast
Li YF(李玉锋); Hu L(胡亮); Li B(李柏); Huang XH(黄小晗); Gao YX(高愈希); Chai ZF(柴之芳); Chen CY(陈春英); Li, YF; Hu, LA; Li, B; Huang, XH; Larsen, EH; Gao, YX; Chai, ZF; Chen, CY
2011
发表期刊JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
卷号26期号:1页码:#REF!
通讯作者Chen, CY (reprint author), Chinese Acad Sci, Inst High Energy Phys, CAS Key Lab Nucl Analyt Tech, Beijing 100049, Peoples R China.
摘要Accurate determination of selenium (Se) species in biological samples is a critical issue because Se commonly occurs at low levels and in diverse species. The method for the full quantification of Se species in serum samples was proposed through combined ion-pair reverse-phase (RP) chromatography and affinity chromatography (AF) hyphenated to inductively coupled plasma( quadrupole) mass spectrometry (ICP-qMS) with post-column isotope dilution analysis (IDA) and a collision cell technique (CCT). Different Se species like inorganic Se (Se4+ and Se6+), selenocystine (SeCys), selenomethionine (SeMet), selenoprotein P (SelP), selenoalbumin (SeAlb) and glutathione peroxidase (GPx) can be separated and quantified. The proposed methodology was used to qualitatively and quantitatively study the dynamic distribution of Se species in human serum samples from the Hg-contaminated area after supplementation with 100 mu g of Se daily as Se-enriched yeast for 180 days. SelP takes up almost half and even more of the total Se and increases with the Se administration. The repeatability in terms of relative standard deviation (R. S. D. %, n = 10) is 6% for GPx and SelP and 5% for SeAlb. The detection limits are 0.1 mu g Se L-1 for GPx and other non-retained Se compounds, 1.0 mu g Se L-1 for SelP and 1.2 mu g Se L-1 for SeAlb, 1.3 mu g Se L-1 for inorganic Se; 1.2 mu g Se L-1 for SeCys; 1.1 mu g Se L-1 for SeMet, respectively.
文章类型Article
学科领域Chemistry; Spectroscopy
DOI10.1039/c0ja00129e
收录类别SCI
WOS类目Chemistry, Analytical ; Spectroscopy
WOS记录号WOS:000285390600025
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/227047
专题院士
实验物理中心
多学科研究中心
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GB/T 7714
Li YF,Hu L,Li B,et al. Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2011,26(1):#REF!.
APA 李玉锋.,胡亮.,李柏.,黄小晗.,高愈希.,...&Chen, CY.(2011).Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast.JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,26(1),#REF!.
MLA 李玉锋,et al."Full quantification of selenium species by RP and AF-ICP-qMS with on-line isotope dilution in serum samples from mercury-exposed people supplemented with selenium-enriched yeast".JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY 26.1(2011):#REF!.
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