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Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells
Liu, Y; Wang, B; Wang, JN; Wan, WZ; Sun, RH; Zhao, YL; Zhang, N; Zhao YL(赵宇亮)
2009
发表期刊LUNG CANCER
卷号63期号:2页码:#REF!
通讯作者Zhang, N (reprint author), Peking Univ, Dept Biol Chem, Beijing Natl Lab Mol Sci, Beijing 100871, Peoples R China.
文章类型Article
摘要Metastasis is the major cause of mortality in lung cancer. Chemotaxis plays a vital role in cancer cell metastasis. In the current study, we reported that epidermal growth factor (EGF) induced a robust chemotaxis of A549 and H1299 cells, two representative human non-small cell lung cancer (NSCLC) cells. Chelerythrine chloride, an inhibitor of all protein kinase C (PKC) isozymes, significantly reduced the chemotactic capacity of NSCLC cells while inhibitors of classical or novel PKC isozymes, such as 666976, calphostin C, or Go6850, showed no effect, which suggested that atypical PKC might be involved in the chemotactic process of NSCLC cells. EGF-elicited translocation and phosphorylation of atypical PKC, indicating that EGF could activate PKC zeta. Treatment with a PKC zeta specific inhibitor, a myristoylated pseudosubstrate, blocked the chemotaxis in a dose-dependent manner, further confirming that atypical PKCI; was required for NSCLC chemotaxis. Mechanistic studies suggested that PKC zeta; was regulated by phosphatidylinositol 3 kinase (PI3K)/Akt. Furthermore, PKC zeta-mediated chemotaxis by regulating actin polymerization and cell adhesion. Taken together, our study suggested that PKC zeta was required in NSCLC cell chemotaxis, thus could be used as a target to develop anti-lung cancer metastasis therapies. (C) 2008 Published by Elsevier Ireland Ltd.
关键词NSCLC PKC zeta Chemotaxis EGFR Metastasis Pseudosubstrate
学科领域Oncology; Respiratory System
DOI10.1016/j.lungcan.2008.05.010
收录类别SCI
WOS类目Oncology ; Respiratory System
WOS记录号WOS:000263020700008
引用统计
被引频次:36[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226963
专题多学科研究中心
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GB/T 7714
Liu, Y,Wang, B,Wang, JN,et al. Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells[J]. LUNG CANCER,2009,63(2):#REF!.
APA Liu, Y.,Wang, B.,Wang, JN.,Wan, WZ.,Sun, RH.,...&赵宇亮.(2009).Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells.LUNG CANCER,63(2),#REF!.
MLA Liu, Y,et al."Down-regulation of PKC zeta expression inhibits chemotaxis signal transduction in human lung cancer cells".LUNG CANCER 63.2(2009):#REF!.
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