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Study of selenium species in selenized rice using high performance liquid chromatography-inductively coupled plasma-mass spectrometry
其他题名高效液相色谱2等离子体质谱联用方法研究富硒大米中硒的形态
Zhang T(张涛); Gao YX(高愈希); Li B(李柏); Li YF(李玉锋); Chen CY(陈春英); Tao, Z; Gan, YX; Bai, L; Li, YF; Cheng, CY; Gang, W
2008
发表期刊CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
卷号36期号:2页码:#REF!
通讯作者Tao, Z (reprint author), Inst High Energy Phys, Lab Biol Effects Nanomat & Nanosafety, Beijing 100049, Peoples R China.
文章类型Article
摘要On the basis of Osborne fractionation, albumin, globulin, prolamine, and gluletin in selenium (Se) rich rice were extracted with water, 2% NaCl solution, 70% alcohol, and 0.5% alkaline solution subsequently. The content of Se in these protein extracts was determined by inductively coupled plasma mass spectrometry (ICP-MS). The chemical forms of Se in albumin extract and enzymatic digests of rice were analyzed with a hyphenated technique of high performance liquid chromatography and ICP-MS. The Se content in four types of extracts shows an order of gluletin > prolamine > albumin > globulin. The protein with molecular weight of 12.6 kDa is the primary Se-containing protein in extracts of albumin, and the Se from selenocysteme is approximately 30% of the total Se present in the selenized rice.
关键词rice selenium speciation analysis high performance liquid chromatography inductively coupled plasma-mass spectrometry
学科领域Chemistry
DOI10.1016/S1872-2040(08)60019-5
收录类别SCI
WOS类目Chemistry, Analytical
WOS记录号WOS:000254252200014
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226933
专题多学科研究中心
实验物理中心
推荐引用方式
GB/T 7714
Zhang T,Gao YX,Li B,et al. Study of selenium species in selenized rice using high performance liquid chromatography-inductively coupled plasma-mass spectrometry[J]. CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,2008,36(2):#REF!.
APA 张涛.,高愈希.,李柏.,李玉锋.,陈春英.,...&Gang, W.(2008).Study of selenium species in selenized rice using high performance liquid chromatography-inductively coupled plasma-mass spectrometry.CHINESE JOURNAL OF ANALYTICAL CHEMISTRY,36(2),#REF!.
MLA 张涛,et al."Study of selenium species in selenized rice using high performance liquid chromatography-inductively coupled plasma-mass spectrometry".CHINESE JOURNAL OF ANALYTICAL CHEMISTRY 36.2(2008):#REF!.
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