Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans
Gao ZQ(高增强); Hou HF(侯海峰); Li LQ(李丽琴); Dong YH(董宇辉); Gao, ZQ; Hou, HF; Li, LQ; Rui, X; Liang, YH; Li, LF; Su, XD; Dong, YH
刊名PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
2007
卷号34期号:2页码:#REF!
关键词Streptococcus mutans Smu_195c crystallization X-ray diffraction
学科分类Biochemistry & Molecular Biology; Biophysics
通讯作者Dong, YH (reprint author), Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China.
文章类型Article
英文摘要Smu_195c is a protein with 86 amino acids in Streptococcus mutans, a primary pathogen for human dental caries. The specific function of Smu_195c is still unknown and there are no conserved domains in it. In Order to find out its function, the gene encodes Smu_195c was cloned and expressed in E. coli as N-terminally 6*His tagged recombinant protein. Two crystal forms were obtained by the hanging drop method. Form I belongs to space group P6(1)22 or P6(5)22 with the unit cell parameters a = b = 62.93 angstrom, c = 90.63 angstrom, gamma = 120 degrees and form II belongs to the space group P4(1)2(1)2 or P4(3)2(1)2 with the unit cell parameters a = b = 57.97 angstrom, c = 103.51 angstrom Crystals from the protein with His-tag belong to form I, however, crystals from the protein without His-tag belong to both.
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
收录类别SCI
WOS记录号WOS:000244317300013
引用统计
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226916
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
推荐引用方式
GB/T 7714
Gao ZQ,Hou HF,Li LQ,et al. Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2007,34(2):#REF!.
APA 高增强.,侯海峰.,李丽琴.,董宇辉.,Gao, ZQ.,...&Dong, YH.(2007).Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,34(2),#REF!.
MLA 高增强,et al."Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 34.2(2007):#REF!.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2205.pdf(205KB)期刊论文作者接受稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[高增强]的文章
[侯海峰]的文章
[李丽琴]的文章
百度学术
百度学术中相似的文章
[高增强]的文章
[侯海峰]的文章
[李丽琴]的文章
必应学术
必应学术中相似的文章
[高增强]的文章
[侯海峰]的文章
[李丽琴]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 2205.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。