Chen, B (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Anal Tech, POB 27327, Beijing 100800, Peoples R China.
The relationship between dose-biological function of selenium and reactive oxygen species (ROS) was investigated both in vitro and in vivo. The formation of ROS as hydroxyl radical was generated in the reaction mixture of sodium selenite, glutathione (GSH), and hydrogen peroxide proved by electron spin resonance (ESR) and spin trapping technique, and lipid radical was also observed by adding linoleic acid to the reaction mixture, and lipid peroxide was elevated by measuring substances reactive to thiobarbituric acid (TBA). Furthermore, some antioxidants and enzymes as superoxide dismutase (SOD) and catalase (CAT) can decrease this elevation. In addition, the experiment animals were administered a Se-deficient diet and drinking water supplemented with different level of selenite for 21 days, and ig CCl4 (0.7 ml/kg) oil solution 10 ml/kg once. After 24 hours the mice were killed and content of Se, glutathione peroxidase (GSH-Px), lipid peroxidation, and free radical level in liver were detected. There are 4 lines gained which agree with Bertrand's rule. Results show that the concentration of Se and its biological activity is closely related to ROS.