Zhang, B (reprint author), Acad Sinica, Inst Microbiol, Dept Microbial Mol Genet & Breeding, Beijing 100080, Peoples R China.
Saccharomyces cerevisiae LZ-53 was selected from 240 primary yeast strains from different genera and species, whose chromium (Cr) resistance and biomass were higher than others were. The highest biomass and Cr content of the strain was obtained in 30 h at 28 degreesC and 200 rpm, when 20 ml of the culture in 250-ml shake flasks was grown in wort containing 1200 mug/ml Cr. The initial pH was adjusted to 6.0. The optimal inoculum volume was 10% (v/v). The Cr content of the cells was determined by neutron activation analysis. Under the optimized cultivation conditions, the Cr content reached 3248 mug/g.
Liu J,ZHANG P,Chai ZF,et al. Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions[J]. JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,2001,27(4):#REF!.
刘景.,ZHANG P.,柴之芳.,Liu, J.,Zhang, B.,...&Chai, Z.(2001).Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions.JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY,27(4),#REF!.
刘景,et al."Selection of a high-biomass, chromium-rich yeast strain and optimization of cultivation conditions".JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY 27.4(2001):#REF!.