Dong, YH (reprint author), Chinese Acad Sci, Inst High Energy Phys, Beijing Synchrotron Radiat Facil, Beijing 100049, Peoples R China.
Streptococcus mutans is one of the pathogenic species involved in dental caries, especially in the initiation and development stages. Here, the crystal structure of SMU.595, a putative dihydroorotate dehydrogenase ( DHOD) from S. mutans, is reported at 2.4 angstrom resolution. DHOD is a flavin mononucleotide-containing enzyme which catalyzes the oxidation of L-dihydroorotate to orotate, which is the fourth step and the only redox reaction in the de novo biosynthesis of pyrimidine nucleotides. The reductive lysine-methylation procedure was applied in order to improve the diffraction qualities of the crystals. Analysis of the S. mutans DHOD crystal structure shows that this enzyme is a class 1A DHOD and also suggests potential sites that could be exploited for the design of highly specific inhibitors using the structure-based chemotherapeutic design technique.
Liu Y,Gao ZQ,Liu CP,et al. Structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2011,67(2):#REF!.
刘颖.,高增强.,刘超培.,徐建华.,董宇辉.,...&Dong, YH.(2011).Structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans.ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,67(2),#REF!.
刘颖,et al."Structure of the putative dihydroorotate dehydrogenase from Streptococcus mutans".ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS 67.2(2011):#REF!.