The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart
Duan, YM; Liu, HT; Zhao, JF; Liu, C; Li, ZR; Yan, JY; Ma, LL; Liu, J; Xie, YN; Ruan, J; Hong, FS; Xie YN(谢亚宁)
刊名BIOLOGICAL TRACE ELEMENT RESEARCH
2009
卷号130期号:2页码:#REF!
关键词Nano-anatase TiO2 Lactate dehydrogenase Enzyme kinetics Conformation
学科分类Biochemistry & Molecular Biology; Endocrinology & Metabolism
DOI10.1007/s12011-009-8326-9
通讯作者Hong, FS (reprint author), Soochow Univ, Coll Med, Suzhou 215123, Peoples R China.
文章类型Article
英文摘要Lactate dehydrogenase (LDH, EC1.1.1.27), widely expressed in the heart, liver, and other tissues, plays an important role in glycolysis and glyconeogenesis. The activity of LDH is often altered upon inflammatory responses in animals. Nano-TiO2 was shown to provoke various inflammatory responses both in rats and mice; however, the molecular mechanism by which TiO2 exerts its toxicity has not been completely understood. In this report, we investigated the mechanisms of nano-anatase TiO2 (5 nm) on LDH activity in vitro. Our results showed that LDH activity was greatly increased by low concentration of nano-anatase TiO2, while it was decreased by high concentration of nano-anatase TiO2. The spectroscopic assays revealed that the nano-anatase TiO2 particles were directly bound to LDH with mole ratio of [nano-anatase TiO2] to [LDH] was 0.12, indicating that each Ti atom was coordinated with five oxygen/nitrogen atoms and a sulfur atoms of amino acid residues with the Ti-O(N) and Ti-S bond lengths of 1.79 and 2.41 angstrom. We postulated that the bound nano-anatase TiO2 altered the secondary structure of LDH, created a new metal ion-active site for LDH, and thereby enhanced LDH activity.
类目[WOS]Biochemistry & Molecular Biology ; Endocrinology & Metabolism
收录类别SCI
WOS记录号WOS:000267102300007
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226602
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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Duan, YM,Liu, HT,Zhao, JF,et al. The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2009,130(2):#REF!.
APA Duan, YM.,Liu, HT.,Zhao, JF.,Liu, C.,Li, ZR.,...&谢亚宁.(2009).The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart.BIOLOGICAL TRACE ELEMENT RESEARCH,130(2),#REF!.
MLA Duan, YM,et al."The Effects of Nano-anatase TiO2 on the Activation of Lactate Dehydrogenase from Rat Heart".BIOLOGICAL TRACE ELEMENT RESEARCH 130.2(2009):#REF!.
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