Wang, KJ (reprint author), ACAD SINICA,INST HIGH ENERGY PHYS,POB 2732,BEIJING 100080,PEOPLES R CHINA.
By following UV absorbance, UV absorption spectra and changes in high performance liquid chromatography hydrate peak elution areas during the reversion of uracil, uridine and 5'UMP hydrates induced by heating hydrated samples in boiling water for 10 min or by incubating acidified samples (pH 1) at room temperature for 24 h, it is shown that these methods do not lead to quantitative dehydration. The disappearance of hydrate peaks and the higher recovery of both the UV absorbance and UV absorption spectra obtained after heating acidified samples (pH 1) indicate that this method is best to achieve total dehydration. Catalysis of dehydration in phosphate buffer solution is also discussed. (C) 1997 Published by Elsevier Science S.A.