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Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence after sodium dodecyl sulphate polyacrylamide gel electrophoresis
Gao YX(高愈希); Chen CY(陈春英); Zhang PQ(章佩群); Chai ZF(柴之芳); He W(何伟); Huang YY(黄宇营); Gao, YX; Chen, CY; Zhang, PQ; Chai, ZF; He, W; Huang, YY
2003
发表期刊ANALYTICA CHIMICA ACTA
卷号485期号:1页码:#REF!
通讯作者Gao, YX (reprint author), Chinese Acad Sci, Inst High Energy Phys, Lab Nucl Analyt Tech, POB 918, Beijing 100039, Peoples R China.
文章类型Article
摘要An improved method of analysis of metals in protein bands with synchrotron radiation X-ray fluorescence (SRXRF) after sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) separation is introduced and applied to human liver cytosol. Through a step of drying the gel before SRXRF determination, the continuous background resulting mainly from the Compton-scattering of X-rays by the gel matrix was substantially reduced, and the detection of biological trace elements, such as Cu, Fe, and Zn in protein bands was thereby made possible. With the new procedure, six Zn-containing proteins with molecular weights (MWs) of 17.5, 20.5, 27, 35, 55, and 63 kDa, respectively were found in human liver cytosol, among which the 63 kDa Zn-containing band was shown to be the dominant form of zinc. In addition, at least four Fe containing proteins with MWs of 20, 23, 43, and 83.5 kDa, respectively, were present in the samples. The metal contents in some metalloproteins, such as the 63 kDa Zn-containing protein, the 23 and 83.5 kDa Fe-containing proteins, and a 22 kDa Cu-containing protein were more closely related to the metal level in the sample. It is demonstrated that the procedure could be widely used to further investigate metal-binding proteins in biological samples. (C) 2003 Elsevier Science B.V. All rights reserved.
关键词synchrotron radiation X-ray fluorescence electrophoresis metal-containing proteins human liver cytosol
学科领域Chemistry
DOI10.1016/S0003-2670(03)00347-7
收录类别SCI
WOS类目Chemistry, Analytical
WOS记录号WOS:000183171900015
引用统计
被引频次:42[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226382
专题院士
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GB/T 7714
Gao YX,Chen CY,Zhang PQ,et al. Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence after sodium dodecyl sulphate polyacrylamide gel electrophoresis[J]. ANALYTICA CHIMICA ACTA,2003,485(1):#REF!.
APA 高愈希.,陈春英.,章佩群.,柴之芳.,何伟.,...&Huang, YY.(2003).Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence after sodium dodecyl sulphate polyacrylamide gel electrophoresis.ANALYTICA CHIMICA ACTA,485(1),#REF!.
MLA 高愈希,et al."Detection of metalloproteins in human liver cytosol by synchrotron radiation X-ray fluorescence after sodium dodecyl sulphate polyacrylamide gel electrophoresis".ANALYTICA CHIMICA ACTA 485.1(2003):#REF!.
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