Study of Pb2+ on the mechanism of trypsin activity
Hong, FS; Wang, L; Wu, K; Wang, XF; Liu, C; Tao, Y; Tao Y(陶冶)
刊名CHINESE JOURNAL OF INORGANIC CHEMISTRY
2003
卷号19期号:2页码:#REF!
关键词trypsin lead ion enzyme activity EXAFS secondary structure
学科分类Chemistry
其他题名Pb2+ 对胰蛋白酶活性影响的作用机理研究
通讯作者Hong, FS (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
文章类型Article
英文摘要The activity of trypsin from bovine pancreas was enhanced under the treatment by Ph2+ at low concentration (0.5 similar to 3 mumol (.) L-1) but was inhibited by Pb2+ at high concentration (3 mumol (.) L-1 above). Pb2+ at high concentration could competitively displace Ca2+ from trypsin. The EXAFS demonstrated that Pb2+ bound to polypeptide chain of trypsin, coordination atom was nitrogen or oxygen, its coordination number was 2 and Pb-N (or 0) bond length was 0.241 nm. The secondary structure of trypsin was greatly changed by Pb2+ at high concentration, e. g. a-helix, beta-turn and Random coil contents decreased and beta-sheet increased. It suggested that Pb2+ bound result in trypsin conformational changed, and enzyme activity decreased.
类目[WOS]Chemistry, Inorganic & Nuclear
收录类别SCI
WOS记录号WOS:000180653400004
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226365
专题中国科学院高能物理研究所_多学科研究中心_期刊论文
中国科学院高能物理研究所_多学科研究中心
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GB/T 7714
Hong, FS,Wang, L,Wu, K,et al. Study of Pb2+ on the mechanism of trypsin activity[J]. CHINESE JOURNAL OF INORGANIC CHEMISTRY,2003,19(2):#REF!.
APA Hong, FS.,Wang, L.,Wu, K.,Wang, XF.,Liu, C.,...&陶冶.(2003).Study of Pb2+ on the mechanism of trypsin activity.CHINESE JOURNAL OF INORGANIC CHEMISTRY,19(2),#REF!.
MLA Hong, FS,et al."Study of Pb2+ on the mechanism of trypsin activity".CHINESE JOURNAL OF INORGANIC CHEMISTRY 19.2(2003):#REF!.
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