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Effect of Pb2+ on RNase activity and its structure
其他题名Pb2+ 对核糖核酸酶活性及其结构的影响
Hong, FS; Wang, L; Wu, K; Wang, XF; Tao, Y; Tao Y(陶冶)
2003
发表期刊ACTA CHIMICA SINICA
卷号61期号:1页码:#REF!
通讯作者Hong, FS (reprint author), Suzhou Univ, Coll Life Sci, Suzhou 215006, Peoples R China.
摘要Activity of RNase from porcine pancreas was enhanced by treatment with Pb2+ at low concentration (0.17 X 10(-2) similar to 0.6 x 10(-2) mumol/L) but was inhibited by Pb2+ - at high concentration (over 0. 85 x 10(-2) mumol/L). Pb2+ at high concentration could competitively displace Ca2+ from RNase and the fluorescence titration showed that one molecule of RNase had three binding sites for Pb2+ the association constant k(sv) for its low-affinity Pb2+-binding site was 6.03 x 10(6)(mol/L) (-1). The extended X-ray absorption fine structure (EXAFS) spectrum demonstrated that Pb2+ coordinated nitrogen or oxygen atoms of the active site of RNase or other amino acid residue of RNase, Pb-N (or O) bond length was 0. 242 nm and 0. 312 nm, and coordination number was 2, respectively. The secondary structure of RNase was greatly damaged by Pb2+ at high concentration resulting in the decrease of alpha-helix, beta-sheet and beta-turn contents and the increase of random coil contents.
文章类型Article
关键词RNase Pb2+ enzyme activity coordination environment secondary structure
学科领域Chemistry
收录类别SCI
WOS类目Chemistry, Multidisciplinary
WOS记录号WOS:000180546100022
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.ihep.ac.cn/handle/311005/226269
专题多学科研究中心
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GB/T 7714
Hong, FS,Wang, L,Wu, K,et al. Effect of Pb2+ on RNase activity and its structure[J]. ACTA CHIMICA SINICA,2003,61(1):#REF!.
APA Hong, FS,Wang, L,Wu, K,Wang, XF,Tao, Y,&陶冶.(2003).Effect of Pb2+ on RNase activity and its structure.ACTA CHIMICA SINICA,61(1),#REF!.
MLA Hong, FS,et al."Effect of Pb2+ on RNase activity and its structure".ACTA CHIMICA SINICA 61.1(2003):#REF!.
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